In vitro inhibition on biofilm production of Candida species by probiotic Lactobacillus strains
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AbstractBackground: Candida biofilms have elevated antifungal resistance and are the possible sources of persistent Candida infections. In this study, we evaluated the inhibitory effects of three Lactobacillus strains on the formation of Candida biofilm.

Materials/methods: Three Lactobacillus reference strains (L. rhamnosus GG ATCC 53103, L. acidophilus ATCC 4356, and L. plantarum ATCC 8014) and seven Candida strains (C. albicans type strain SC5314, and 2 bloodstream isolates of each species of C. albicans, C. tropicalis and C. parapsilosis) were used in this study. Biofilm formation was performed using a 96-wells plate system. Candida cells (1x106 CFU in RPMI 1640) were first incubated in the wells for 90 minutes. Non-adherent cells were then removed by washing and culture medium replenished. Cell suspensions or cell-free supernatants (CFS) of the Lactobacillus strains were added to these wells. MRS broth was added as a control. The biofilms were incubated for 24 hours before being assessed by crystal violet staining and XTT reduction assay. Results of the experiment groups were expressed as a percentage of the control group and compared to the control using Student’s t test.

Results: Cell suspensions of the Lactobacillus strains had little to none inhibitory effect on the biofilm formation, whereas CFS of L. rhamnosus GG (LGG) significantly reduced the biomass production and metabolic activities of C. albicans and C. tropicalis strains. The CFS of L. plantarum ATCC 8014 (Lp8014) also exhibited inhibitory effect, but to a lesser degree. In contrast, the C. parapsilosis strains were insensitive to CFS of LGG and Lp8014 but showed significant inhibition when incubated with cell suspension or CFS of L. acidophilus ATCC 4356 (La4356).

Conclusions: Our study demonstrated that CFSs of LGG and Lp8014 cultures possessed inhibitory effects on biofilm formation of C. albicans and C. tropicalis, whilst La4356 could inhibit C. parapsilosis biofilm by its cell suspension and CFS.
Acceptance Date21/01/2019
All Author(s) ListY Poon, M Hui
Name of Conference29th European Congress of Clinical Microbiology & Infectious Diseases
Start Date of Conference13/04/2019
End Date of Conference16/04/2019
Place of ConferenceAmsterdam
Country/Region of ConferenceNetherlands
Year2019
LanguagesEnglish-United Kingdom

Last updated on 2020-02-03 at 11:52