Biofilm production among bloodstream isolates of Candida albicans, C. tropicalis and C. parapsilosis
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摘要Background: Biofilm production is recognized as a virulence factor of Candida species and is associated with catheter-related candidemia. The aim of our study was to evaluate biofilm production of bloodstream isolates of Candida albicans, C. tropicalis and C. parapsilosis.

Materials/methods: Biofilm production was analysed using a 96-well plate-based method. Two assays, crystal violet (CV) staining and XTT reduction assay, were used to quantify the biomass and the metabolic activity of 24 hour-old biofilms. Candida strains were categorised into weak, moderate or strong biofilm producers based on the optical density (OD) at 570 nm in CV assay. Biofilm properties were compared among Candida strains using Kruskal–Wallis test.

Results: A total of 96 blood isolates (C. albicans n=48, C. tropicalis n=32, and C. parapsilosis n=16) collected during 2013 to 2017 were screened. Forty-eight percent of C. albicans (n=23), 75% of C. tropicalis (n=24), and 69% of C. parapsilosis (n=11) strains were classified as moderate or strong biofilm formers. Biofilm produced by C. tropicalis had significantly more biomass than C. albicans and higher metabolic activity than C. albicans and C. parapsilosis (p < 0.001). Metabolic activity of biofilm was higher in C. albicans when compared to C. parapsilosis (p = 0.032).

Conclusions: Biofilm production varied among species and isolates. In our study, C. tropicalis isolates were more likely to produce biofilm with more biofilm mass and higher metabolic activity. We also observed that the levels of biomass and metabolic activity of the biofilm were not always correlated. Strains and detection methods should be selected according to the biofilm properties of interest.
著者Y Poon, M Hui
會議名稱29th European Congress of Clinical Microbiology & Infectious Diseases

上次更新時間 2020-02-03 於 11:45