Methylation analysis of plasma DNA informs etiologies of Epstein-Barr virus-associated diseases
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AbstractEpstein-Barr virus (EBV) is associated with a number of diseases, including malignancies. Currently, it is not known whether patients with different EBV-associated diseases have different methylation profiles of circulating EBV DNA. Through whole-genome methylation analysis of plasma samples from patients with nasopharyngeal carcinoma (NPC), EBV-associated lymphoma and infectious mononucleosis, we demonstrate that EBV DNA methylation profiles exhibit a disease-associated pattern. This observation implies a significant potential for the development of methylation analysis of plasma EBV DNA for NPC diagnostics. We further analyse the plasma EBV DNA methylome of NPC and non-NPC subjects from a prospective screening cohort. Plasma EBV DNA fragments demonstrate differential methylation patterns between NPC and non-NPC subjects. Combining such differential methylation patterns with the fractional concentration (count) and size of plasma EBV DNA, population screening of NPC is performed with an improved positive predictive value of 35.1%, compared to a count- and size-based only protocol.
All Author(s) ListLam WKJ, Jiang PY, Chan KCA, Peng WL, Shang HM, Heung MMS, Cheng SH, Zhang HQ, Tse OYO, Raghupathy R, Ma BBY, Hui EP, Chan ATC, Woo JKS, Chiu RWK, Lo YMD
Journal nameNature Communications
Year2019
Month7
Day22
Volume Number10
PublisherNature Research (part of Springer Nature): Fully open access journals / Nature Publishing Group
Article number3256
ISSN2041-1723
LanguagesEnglish-United Kingdom
Web of Science Subject CategoriesMultidisciplinary Sciences;Science & Technology - Other Topics

Last updated on 2021-04-12 at 00:46