Aleuritolic Acid Impaired Autophagic Flux and Induced Apoptosis in Hepatocellular Carcinoma HepG2 Cells
Publication in refereed journal

替代計量分析
.

其它資訊
摘要Aleuritolic acid (AA) is a triterpene that is isolated from the root of Croton crassifolius Geisel. In the present study, the cytotoxic effects of AA on hepatocellular carcinoma cells were evaluated. AA exerted dose- and time-dependent cytotoxicity by inducing mitochondria-dependent apoptosis in the hepatocellular carcinoma cell line, HepG2. Meanwhile, treatment with AA also caused dysregulation of autophagy, as evidenced by enhanced conversion of LC3-I to LC3-II, p62 accumulation, and co-localization of GFP and mCherry-tagged LC3 puncta. Notably, blockage of autophagosome formation by ATG5 knockdown or inhibitors of phosphatidylinositol 3-kinase (3-MA or Ly294002), significantly reversed AA-mediated cytotoxicity. These data indicated that AA retarded the clearance of autophagic cargos, resulting in the production of cytotoxic factors and led to apoptosis in hepatocellular carcinoma cells.
著者Yi H, Wang K, Du BY, He LN, Ho HT, Qiu MS, Zou YD, Li Q, Jin JF, Zhan YJ, Zhao ZX, Liu XD
期刊名稱Molecules
出版年份2018
月份6
卷號23
期次6
出版社MDPI
文章號碼1338
國際標準期刊號1420-3049
語言英式英語
關鍵詞aleuritolic acid, autophagy, apoptosis
Web of Science 學科類別Biochemistry & Molecular Biology;Chemistry, Multidisciplinary;Biochemistry & Molecular Biology;Chemistry

上次更新時間 2021-21-01 於 02:22