High-Throughput Immunological Analysis of Dictamni Cortex: Implication in the Quality Control of Herbal Medicine
Publication in refereed journal

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摘要Quality inconsistency of herbal medicine is an obstacle that limits the extensive use and study of traditional Chinese medicine. Differences in environmental conditions and processing methods of herbal medicine often result in varying clinical outcomes in patients. Standard chemical markers used for the quality control (QC) of herbal medicine are usually the most abundant and characteristic components, which may not be therapeutically relevant or cannot comprehensively reflect the biological quality of the herbs. In view of this, a novel QC method for better assessment of herbal medicine has been developed via bioactivities analysis. Immunological activities of Dictamni Cortex, a typical herbal medicine for the treatment of various inflammatory diseases, from different geographical locations in China, were evaluated. Upon in vitro treatment of their water and ethanol extracts, distinct patterns of inflammatory cytokines including tumor necrosis factor (TNF)-α, interleukin (IL)-10, IL-6, IL-12p70, IL-1β, and chemokine CXCL8 were released from the lipopolysaccharides- and/or phytohaemagglutinin-stimulated human peripheral blood mononuclear cells (PBMC). Thus, in addition to the commonly used morphological, chemical, or DNA markers, the novel high-throughput profiling of inflammatory cytokines and chemokines of PBMC upon treatment with herbal extracts could be an important reference to help for the quality control of herbal medicine in the future.
出版社接受日期07.08.2019
著者Miranda Sin-Man Tsang, Pang-Chui Shaw, Ida Miu-Ting Chu, Ling Cheng, Eric Chun-Wai Wong, David Tai-Wai Lau, Christopher Wai-Kei Lam, Chun-Kwok Wong
期刊名稱Molecules
出版年份2019
月份8
日期8
卷號24
期次16
出版社MDPI AG
文章號碼2880
頁次1 - 16
國際標準期刊號1420-3049
語言美式英語
關鍵詞cytokines; herbal medicine; immune profiles; quality control

上次更新時間 2020-15-10 於 03:18