Compressive sensing for fast 3-D and random-access two-photon microscopy
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Abstract3-D two-photon excitation (TPE) microscopy has been a critical tool for biological study since its introduction. Yet, the speed is largely limited by its point detector, e.g. photomultiplier tube (PMT), which requires a point-scanning imaging sequence. In this letter, we present a multi-focus compressive sensing (CS) method for 3-D and random-access TPE microscopy based on a digital micromirror device (DMD). This new platform combines compressive sensing with a unique holography-based DMD random-access scanner to enhance the imaging speed by 3-5 times for imaging arbitrarily selected regions in 3-D specimens without sacrificing the resolution. In the experiments, 1 - 20 randomly selected foci are generated by modulating the wavefront of a femtosecond laser via binary holography, where the combined intensity is recorded by a PMT. By exploiting compressive sensing algorithms, 3-D images at arbitrarily selected sites can be reconstructed. Simulations and imaging experiments on different samples have been performed to verify the principle and identify the optimal processing parameters, including number of laser foci and sampling ratio. The results show that high resolution images can be obtained by using a 25% sampling ratio and 5 foci. The new compressive sensing-based TPE imaging method may find important applications in biological studies, e.g., neuronal imaging and optogenetics.
Acceptance Date24/07/2019
All Author(s) ListChenyang Wen, Mindan Ren, Fu Feng, Wang Chen, Shih-Chi Chen
Journal nameOptics Letters
Volume Number44
Issue Number17
Place of PublicationUSA
Pages4343 - 4346
LanguagesEnglish-United States
Keywordscompressive sensing, two-photon microscopy

Last updated on 2020-21-10 at 02:10