Low-magnitude high-frequency vibration enhanced osteoporotic fracture healing in the presence of sarcopenia
Refereed conference paper presented and published in conference proceedings

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AbstractIntroduction: Delayed osteoporotic fracture healing was found in sarcopenic SAMP8 compared with the control SAMR1. LMHFV could enhance osteoporotic fracture healing. Myostatin was a key mediator in sarcopenia and fracture healing. In this study, we evaluated the efficacy of LMHFV on osteoporotic fracture healing with sarcopenia and investigated the role of myostatin played in this process.
Methodology: The in vivo part, closed fracture was created at the right femur of 8-month-old SAMP8 and SAMR1. Control groups (SAMP8, SAMR1) received no treatment; those in LMHFV groups (SAMP8-V, SAMR1-V) received daily LMHFV treatment. Radiographs, Micro CT, histology, mechanical testing, myostatin expression were performed at selected timepoints. The in vitro part, conditional medium of myofibers from aged and young SAMP8 were cultured without LMHFV treatment was further used for ontogenetic MC3T3 with or without myostatin inhibitor followed by alkaline phosphatase staining, quantitative real time PCR and western blot of Runx2.
Results and Analysis: At week 2, Callus formation was enhanced in SAMP8-V group than SAMP8, despite not significantly and significantly enhanced in SAMR1-V as compared to SAMR1. At week 4, SAMP8 showed significantly poorer mechanical properties than SAMR1 and the mechanical properties were significantly enhanced in SAMP8-V. At Week 2, myostatin expression of SAMP8 was significantly higher compared to the other groups. ALP staining and Runx2 was lower in aged group compared to the young groups, and up-regulated after treated with myostatin inhibitor.
Discussion and Conclusion: LMHFV could partly enhance the delayed osteoporotic fracture healing in the presence of
sarcopenia partly by blocking the myostatin pathways.
Acceptance Date31/07/2018
All Author(s) ListSKH Chow, N Zhang, KS Leung, WH Cheung
Name of ConferenceThe 38th Annual Congress of Hong Kong Orthopaedic Association
Start Date of Conference03/11/2018
End Date of Conference04/11/2018
Place of ConferenceHong Kong
Country/Region of ConferenceHong Kong
LanguagesEnglish-United States

Last updated on 2019-03-12 at 15:55