Low-magnitude high-frequency vibration treatment and magnesium enhance myotube formation in myoblasts – an implication on sarcopenia
Refereed conference paper presented and published in conference proceedings


摘要Introduction: Sarcopenia is an age-related geriatric syndrome leading to generalized loss of muscle mass and strength with a risk of adverse outcomes. Low-magnitude high-frequency vibration (LMHFV) is a non-invasive biophysical intervention providing systemic mechanical stimulation. Meanwhile, aging is associated with magnesium deficiency and dietary magnesium (Mg) was reported to help conservation of age-related loss of skeletal mass and power in elderly. The study aims to investigate the effects of combined applications of LMHFV and Mg on myotube hypertrophy in myoblast cell line and look into the mechanism of PI3K/Akt/mTOR pathway.

Methodology: C2C12 myoblasts were fused into myotubes at 70% confluence in differentiation medium. The time point at which differentiation was induced was regarded as day 0 (D0). 10mM Mg and LMHFV (35 Hz, 0.3g; 20min/day) were
administered on D1, while PI3k-Akt/mTOR inhibitors were on D3. Myotube formation was examined by immunofluorescence on D5. The transcriptional expression levels of myogenic regulatory factors, MAFbx and MuRF1 were assessed by qPCR; the translational level of PI3k p85, pAkt, mTOR were detected by Western Blot.

Results and Analysis: By calculating infusion Index and myotube diameter, myosin heavy chain (MHC) type IIa expressions in Mg group and LMHFV group were respectively 2.1X (p< 0.01) and 2.3X (p<0.001) higher than control group. In contrast, with the inhibition of PI3k-Akt/mTOR, myotube formation was partially abolished, which western blot results further substantiated the findings.

Discussion and Conclusion: Mg and vibration separately could accelerate and increase myotube formation compared to control group through the PI3k-akt/mTOR pathway; however, there was no synergistic effect detected for combined
著者SKH Chow, C Cui, L Qin, N Zhang, JY Wang, WH Cheung
會議名稱The 38th Annual Congress of Hong Kong Orthopaedic Association
會議地點Hong Kong

上次更新時間 2019-03-12 於 15:50