Overexpression of mechanical sensitive miR-337-3p alleviates ectopic ossification in rat tendinopathy model via targeting IRS1 and Nox4 of tendon derived stem cells
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摘要Tendinopathy, which is characterized by the ectopic ossification of tendon, is a common disease occurred in certain population, such as athletes that suffer from repetitive tendon strains. However, the molecular mechanism underlying the pathogenesis of tendinopathy caused by overuse of tendon is still lacking. Here, we found that the mechanosensitive miRNA, miR-337-3p, reduced its expression under uniaxial cyclical mechanical loading in tendon-derived stem cells (TDSCs) and negatively controlled chondro-osteogenic differentiation of TDSCs. Importantly, downregulation of miR-337-3p expression was also observed in both rat and human calcified tendons, and overexpressing miR-337-3p in patellar tendons of rat tendinopathy model displayed a robust therapeutic efficiency. Mechanistically, we found that the proinflammatory cytokine Interleukin-1β (IL-1β) was the upstream factor of miR-337-3p that bridges the mechanical loading with its downregulation. Furthermore, the target genes of miR-337-3p, NADPH oxidase 4 (Nox4) and insulin receptor substrate 1 (IRS1), activated chondro-osteogenic differentiation of TDSCs through JNK and ERK signaling, respectively. Thus, these findings not only provide novel insight into the molecular mechanisms underlying ectopic ossification in tendinopathy but also highlight the significance of miR-337-3p as a putative therapeutic target for clinic treatment of tendinopathy.
著者Geng, Zhao, Xu, Zhang, Hu, Fu, Dai, Chen, Patrick, Zhang
期刊名稱Journal of molecular cell biology
出版年份2019
月份5
出版社Oxford University Press
國際標準期刊號1674-2788
語言英式英語
關鍵詞mechanosensitive miRNA, tendon-derived stem cells, mechanical loading, chondro-osteogenesis, tendinopathy

上次更新時間 2020-21-10 於 02:58