A New Reproducible and Clinically Relevant Metaphyseal Fracture Model in Osteoporotic Rats to Study Enhancement of Bone Healing
Refereed conference paper presented and published in conference proceedings


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AbstractIntroduction: Most osteoporotic fractures occur at the metaphyseal bone region but few models exist and the healing is poorly understood. There are 2 purposes to the current study 1) to conduct a systematic review to analyze current osteoporotic metaphyseal fracture animal models 2) to create a new osteoporotic metaphyseal fracture model Materials and Methods: Literature search was performed on the Pubmed, Embase and Web of Science databases and relevant articles were reviewed. For the creation of the model, 26 6-month old Sprague-Dawley rats were obtained. All rats underwent induction of osteoporosis. A 6-hole T-shaped mini-plate and screws were used to fix the plate to the distal femur and an osteotomy was performed. X-ray of the femur was taken weekly and the rats were euthanized at 1, 2, 4 and 6 weeks post-surgery. For analysis, sections cut longitudinally were stained with Hematoxylin-Eosin. Results: A total of 19 studies were included in the systematic review. We propose that an animal model should satisfy the following criteria: 1) induction of osteoporosis, 2) unilateral complete osteotomy or defect at the metaphysis and 3) internal fixation. There was complete bony bridging in all animals at 6 weeks. Intramembranous ossification was observed during the healing phase. Discussion and conclusion: Upon detailed analysis of the current models we have derived a recommendation and created a valid and reproducible metaphyseal fracture model that satisfies these criteria, which can provide a platform for future quality studies to enhance osteoporotic fracture healing. Acknowledgement: Health and Medical Research Fund (Ref: 04152406)
All Author(s) ListWong Ronald Man Yeung, Choy Man Huen Victoria, Leung Kwok Sui, Chow Simon Kwoon Ho, Cheung Wing Hoi, Cheng Jack Chun Yiu
Name of ConferenceThe 12th International Congress Of Chinese Orthopaedic Association
Start Date of Conference15/11/2017
End Date of Conference18/11/2017
Place of ConferenceZhuhai
Country/Region of ConferenceChina
Year2017
LanguagesEnglish-United Kingdom

Last updated on 2019-03-12 at 14:44