Norovirus Virus-like Particles - Good Surrogates for Native Virions in Binding Analyses?
Refereed conference paper presented and published in conference proceedings


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AbstractIntroduction
Human norovirus is a leading cause of acute gastroenteritis. Virus-like particles (VLPs) have been widely used in norovirus studies because they are structurally resembling to native viruses and exhibit authentic conformation of important antigenic epitopes. However, direct side-by-side comparison of VLPs with native virions remains unexplored.

Aim
To compare the binding pattern of two types of VLPs and the corresponding native virus.

Methods
VLPs of a norovirus GII.Pe-GII.4 Sydney strain (CUHK-NS-141) was expressed in a baculovirus system. Two forms of VLPs were expressed: one with only the major capsid protein VP1 (denoted as VLP1) and another with both major (VP1) and minor (VP2) capsid proteins (denoted as VLP1+2). Binding patterns of the two types of VLPs and the corresponding native virus (in stool filtrate) to saliva, histo-blood group antigens, and human intestinal tissues were assayed.

Results
In saliva binding assay, VLP1+2 bound strongly to secretors of blood groups A and AB and to a lesser extent to that of B; whereas native virus and VLP1 only bound weakly to secretor. In HBGAs binding assay, VLP1+2 bound strongly to A and H antigens and to a lesser extent to B antigen, similar to that in saliva binding assay. Native virus only bound weakly to A antigen. In human tissue binding assay, VLP1+2, VLP1, and native virus bound in an indistinguishable pattern to a small subset of cells in lamina propria of duodenum. No binding to epithelial surface was observed. In stomach, VLP1+2 bound strongly to lamina propria; however, no binding was observed for VLP1 and native virus.

Conclusion
In conclusion, norovirus VLPs are good surrogates for native norovirus only when used on small intestinal tissue. The ability that VLPs “can” bind to saliva and carbohydrates do not mean that native virus “will” bind to these well-known attachment factors. (GRF 14101314)
Acceptance Date03/04/2019
All Author(s) ListMartin Chi-Wai Chan, Sarah K.C. Cheung
Name of ConferenceAmerican Society for Virology's 38th Annual meeting (ASV 2019)
Start Date of Conference20/07/2019
End Date of Conference24/07/2019
Place of ConferenceTwin Cities
Country/Region of ConferenceUnited States of America
Year2019
Month7
LanguagesEnglish-United States
Keywordsnorovirus, virus-like particles, HBGAs, saliva, gastroenteritis

Last updated on 2019-28-11 at 10:20