Photodynamic therapy of BAM-SiPc, a silicon (IV) phthalocyanine derivative, induces immunogenic cell death
Refereed conference paper presented and published in conference proceedings

香港中文大學研究人員

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摘要Photodynamic therapy (PDT), a kind of cancer treatment, is a process using three individually non-toxic substances including a photosensitizer, light and oxygen to produce a toxic effect (reactive oxygen species). It eradicates the tumor by killing localized tumor cells and triggering damage of blood vessels which supply oxygen and nutrients. In addition, there is evidence showing that PDT can trigger anti-tumor immunity and prevent tumor relapse but the mechanism is unclear. As most of the cancer patients die with cancer metastasis and relapse rather than localized tumor, anti-tumor immunity appears to be of great importance. Immunogenic cell death, together with the damage-associated molecular patterns such as calreticulin (CRT) and ATP released from dying tumor cells, can trigger the maturation of dendritic cells (DCs) to activate immune system. In our previous study, we have synthesized a photosensitizer, BAM-SiPc, which can induce the translocation of CRT to cell membrane and protect mice from tumor relapse. With the use of specific inhibitors and co-immunoprecipitation, we have confirmed that BAM-SiPc-PDT could induce cell death through necroptosis (a kind of immunogenic cell death). At the same time, BAM-SiPc-PDT induces the translocation of CRT to cell surface and the release of ATP to the culture medium. After co-culturing with DCs, we found that the post-PDT cells can induce the maturation of DCs with respect to both phenotype (increased relative expression of MHC II, CD80 and CD86 on cell surface) and function (increased release of interleukin 12).
著者ZHANG Ying, FONG Wing Ping
會議名稱10th European Immunology Conference
會議開始日13.06.2019
會議完結日14.06.2019
會議地點Berlin
會議國家/地區德國
會議論文集題名Journal of Clinical and Cellular Immunology
出版年份2019
月份6
卷號10
國際標準期刊號2155-9899
語言英式英語
關鍵詞photodynamic therapy, photosensitizer, immunogenic cell death

上次更新時間 2019-27-09 於 10:38