Photodynamic therapy of BAM-SiPc, a silicon (IV) phthalocyanine derivative, induces immunogenic cell death
Refereed conference paper presented and published in conference proceedings



摘要Photodynamic therapy (PDT), a kind of cancer treatment, is a process using three individually non-toxic substances including a photosensitizer, light and oxygen to produce a toxic effect (reactive oxygen species). It eradicates the tumor by killing localized tumor cells and triggering damage of blood vessels which supply oxygen and nutrients. In addition, there is evidence showing that PDT can trigger anti-tumor immunity and prevent tumor relapse but the mechanism is unclear. As most of the cancer patients die with cancer metastasis and relapse rather than localized tumor, anti-tumor immunity appears to be of great importance. Immunogenic cell death, together with the damage-associated molecular patterns such as calreticulin (CRT) and ATP released from dying tumor cells, can trigger the maturation of dendritic cells (DCs) to activate immune system. In our previous study, we have synthesized a photosensitizer, BAM-SiPc, which can induce the translocation of CRT to cell membrane and protect mice from tumor relapse. With the use of specific inhibitors and co-immunoprecipitation, we have confirmed that BAM-SiPc-PDT could induce cell death through necroptosis (a kind of immunogenic cell death). At the same time, BAM-SiPc-PDT induces the translocation of CRT to cell surface and the release of ATP to the culture medium. After co-culturing with DCs, we found that the post-PDT cells can induce the maturation of DCs with respect to both phenotype (increased relative expression of MHC II, CD80 and CD86 on cell surface) and function (increased release of interleukin 12).
著者ZHANG Ying, FONG Wing Ping
會議名稱10th European Immunology Conference
會議論文集題名Journal of Clinical and Cellular Immunology
關鍵詞photodynamic therapy, photosensitizer, immunogenic cell death

上次更新時間 2019-27-09 於 10:38