Sulforaphane ameliorates the social impairments by normalizing GABA receptors and NMDA receptors expressions in the cerebellum of valporate-induced autistic mouse model
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AbstractBackground and Aims:
Autism spectrum disorders (ASD), is a neurodevelopmental disorder, characterized with impairment in social communication; together with restricted and repetitive behaviors (RRB). With a rising trend, by 2014, 0.7% of children aged below 15 years had been diagnosed with autism by professionals in Hong Kong. So far, there is not any prescribed medicine for ASD patients. Sulforaphane (SFN) is a low toxic natural product, derived from broccoli and Raphani Semen. SFN enriched extract has been reported to improve social interaction on young autistic men in clinical trial, suggesting SFN may be effective in treating autism. However, the mechanism has not been explained clearly. Previous study has reported that valporic acid (VPA) -induced mice have social impairment. In this project, VPA-induced mice model was used to study the mechanism of SFN on treatment of autism in vivo.
Pregnant BALB/c mice were injected with VPA on E12.5 to induce autism. The offspring they had autistic behaviour. The autistic mice were divided into two groups on PND 28: VPA group treated with saline; SFN group treated with SFN. The social behavioral test was conduct once a week. All mice were euthanized on PND49 and their brains were harvested for immunohistochemistry analysis and quantitative PCR (qPCR) test.
SFN could improve the sociability and social novelty in VPA-treated mice. Creysl violet stained sections demonstrated that VPA control group had fewer Purkinje cells (PCs) in the cerebellum as compared with the PBS group. However, SFN treatment group could significantly increase the PCs number as compared with VPA group, nearly similar to PBS group. SFN could markedly up-regulated the mRNA expression of inhibitory neurotransmitter receptor subunit in GABRA5 and GABRA3, while down-regulated excitatory neurotransmitter receptor subunit NR2A and NR2C in cerebellum of autistic mouse, as compared with the VPA control group.
Sulforaphane could ameliorate the social impairment induced by VPA in mice. It action mechanism may be mediate, at least in part, by restoring the loss of PCs number, and stimulating the mRNA expression of GABAergic receptors and NMDA receptors in the cerebellum of VPA-treated mice.
All Author(s) ListWen Yang, Davor Kin-Fan Chau, Angus Yiu-Ting Choi, Chun Wai Chan, Yanfang Xian, Zhixiu Lin
Name of ConferenceInternational Conference on Brain Research in Chinese Medicine: Degeneration and Repair
Start Date of Conference26/07/2018
End Date of Conference28/07/2018
Place of ConferenceHong Kong
Country/Region of ConferenceHong Kong
Proceedings TitleInternational Conference on Brain Research in Chinese Medicine: Degeneration and Repair
LanguagesEnglish-United Kingdom

Last updated on 2019-24-10 at 11:31