Eukaryotic elongation factor-2 kinase expression is an independent prognostic factor in colorectal cancer
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AbstractBackground
Prognostication of patients with colorectal cancer (CRC) currently relies on tumor-node-metastasis (TNM) staging but clinical outcomes of patients of the same histoclinical stage are heterogeneous. It is therefore imperative to devise novel molecular tests to stratify CRC patients. Our previous work demonstrated that eukaryotic elongation factor-2 kinase (EEF2K) is a tumor suppressor in CRC. Herein, we investigated EEF2K expression in CRC and determined its relationship with clinicopathological parameters.

Methods
Quantitative RT-PCR and Westerns blots were used to examine EEF2K expression in primary tumor and the adjacent non-tumor tissues of CRC patients (n = 20). Kaplan-Meier curves and Cox regression analysis were used to assess the association between clinical outcomes of CRC patients and EEF2K protein expression determined by immunohistochemistry on tissue microarray (n = 151).

Results
EEF2K was significantly downregulated at both mRNA and protein levels in tumors of CRC patients. Univariate Cox regression analysis revealed that CRC patients with high tumor grade, advanced TNM staging and low EEF2K expression were associated with worse overall survival. Multivariate analysis further demonstrated that low EEF2K expression was an independent factor for predicting poorer overall survival in CRC patients (p = 0.014; Hazard ratio = 2.951; 95% confidence interval: 1.240–7.024). The 5-year survival rate was 82.8% in the EEF2K-high-expression group versus 63.9% in the EEF2K-low-expression group (p = 0.0118). The association of overall survival with EEF2K expression in CRC patients was verified in The Cancer Genome Atlas (TCGA) cohort.

Conclusions
EEF2K is downregulated in CRC and its expression can be employed as a prognostic marker for CRC patients independent of TNM staging.
All Author(s) ListTung H. NG, Kathy W. Y. SHAM, Chuan M. XIE, Simon S. M. NG, Ka F. TO, Joanna H. M. TONG, Wing Y. Z. LIU, Lin ZHANG, Matthew T. V. CHAN, William K. K. WU, Christopher H. K. CHENG
Journal nameBMC Cancer
Year2019
Month7
Day2
Volume Number19
Issue Number1
Article number649
ISSN1471-2407
LanguagesEnglish-United Kingdom
KeywordsEEF2K, Colon cancer, Survival, TNM staging, Biomarker

Last updated on 2020-27-06 at 01:05