Systematic Evaluation and Optimization on the Experimental Steps in RNA G-quadruplex Structure Sequencing
Refereed conference paper presented and published in conference proceedings

香港中文大學研究人員

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摘要Guanine (G)-rich sequences in RNA can fold into stacks of G-quartets, and assembly into secondary structure called RNA G-quadruplex (rG4). To map the rG4s in a high-throughput and transcriptome-wide manner, we have previously developed RNA G-quadruplex Structure Sequencing (rG4-seq). Yet, its current limitations to further utilities are i) high RNA input needed, ii) long preparation time required, and iii) lack of a robust experimental pipeline. Here, we evaluate and optimize the 5 key steps involved (See scheme). Overall, we have lowered the RNA input and shortened the preparation time. This improved method can be applied to the structurome studies and other biological applications that require cDNA library preparation in a more efficient manner.
出版社接受日期10.04.2019
著者Pui Yan Yeung, Jieyu Zhao, Eugene Yui-Ching Chow, Huiqi Hong, Leilei Chen, Ting-Fung Chan, Chun Kit Kwok
會議名稱24th Annual Meeting of the RNA Society 2019
會議開始日11.06.2019
會議完結日16.06.2019
會議地點Krakow, Poland
會議國家/地區波蘭
出版年份2019
語言美式英語

上次更新時間 2019-21-10 於 16:10