Potential Roles of the Retinoblastoma Protein in Regulating Genome Editing
Publication in refereed journal

香港中文大學研究人員

引用次數
替代計量分析
.

其它資訊
摘要Genome editing is an important tool for modifying genomic DNA through introducing DNA insertion or deletion at specific locations of a genome. Recently CRISPR/Cas9 has been widely employed to improve the efficiency of genome editing. The Cas9 nuclease creates site-specific double strand breaks (DSBs) at targeted loci in the genome. Subsequently, the DSBs are repaired by two pathways: Homologous Recombination (HR) and Non-Homologous End-Joining (NHEJ). HR has been considered as "error-free" because it repairs DSBs by copying DNA sequences from a homologous DNA template, while NHEJ is "error-prone" as there are base deletions or insertions at the breakage site. Recently, RB1, a gene that is commonly mutated in retinoblastoma, has been reported to affect the repair efficiencies of HR and NHEJ. This review focuses on the roles of RB1 in repairing DNA DSBs, which have impacts on the precision and consequences of the genome editing, both at the targeted loci and the overall genome.
著者Jiang YN, Chu WK
期刊名稱FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
出版年份2018
月份7
卷號6
出版社FRONTIERS MEDIA SA
文章號碼UNSP 81
國際標準期刊號2296-634X
語言英式英語
關鍵詞retinoblastoma, genome editing, CRISPR/Cas9, homologous recombination, Non-Homologous End-Joining
Web of Science 學科類別Cell Biology;Developmental Biology;Cell Biology;Developmental Biology

上次更新時間 2021-17-06 於 00:40