Notch Signaling Activation Enhances Human Adipose-Derived Stem Cell Retinal Differentiation
Publication in refereed journal

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其它資訊
摘要Retinal disease treatment by stem cell-based replacement relies on stem cell differentiation into retinal cells. We previously demonstrated that human periodontal ligament-derived stem cells can be directed into retinal lineage upon induction. Here, we report the transdifferentiation potential of human adipose-derived stem cells (ASCs) into retinal lineage and its enhancement by Notch signaling modulation. Human ASCs, isolated from abdominal fat, expressed mesenchymal but not hematopoietic stem cell markers, and they can differentiate into adipocytes, chondrocytes, and osteoblasts in vitro. Upon noggin/Dkk-1/IGF-1 induction, the treated ASCs showed elevated expression of retinal progenitor, retinal ganglion, and photoreceptor cell markers as well as the glutamate-evoked calcium response, which was not observed in the noninduced cells. Compared to the regular induction treatment, Notch signaling activation by JAG1 enhanced the expression of retinal progenitor and precursor markers without affecting the glutamate-evoked calcium response. In contrast, Notch signaling inhibition by DAPT showed more retinal ganglion cells, but delayed the response to glutamate stimulation. In summary, our results revealed that human ASCs possess a retinal transdifferentiation potential upon noggin/Dkk-1/IGF-1 induction, which can further be enhanced by Notch signaling activation.
著者Huang YQ, Ng TK, Chen CB, Huang B, Liang JJ, Pang CP, Zhang MZ
期刊名稱Stem Cells International
出版年份2018
月份10
卷號2018
出版社HINDAWI LTD
文章號碼9201374
國際標準期刊號1687-966X
電子國際標準期刊號1687-9678
語言英式英語
Web of Science 學科類別Cell & Tissue Engineering;Cell Biology

上次更新時間 2021-18-01 於 01:37