Production of mushroom β-glucan-gold nanorod biohybrids for photothermal therapy of cancer
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摘要Photothermal therapy (PTT) involves the conversion of absorbed light into local heating through non-radiative mechanism for non-invasive cancer cell ablation fast. Surface plasmon resonant (SPR) gold nanorods (AuNRs) have been investigated for near-infrared (NIR) PTT using ~1064 nm laser. However, clinical application of AuNRs are restricted due to their instability in gastrointestinal tract (GIT) and cytotoxicity caused by the residual positively charged cetyltrimethylammonium bromide (CTAB), which is the essential structural template and surface ligand for AuNRs. Natural β-glucan extracted from mushroom sclerotia of Pleurotus tuber-regium (PTR) has a hyper-branched structure with a spherical architecture that can facilitate the protection of metal nanoparticles in a shell structure to be delivered to the colon.
The objective of this study was to produce a stable and biocompatible mushroom β-glucan encapsulated gold nanorod hybrids (AuNR-Glu) by integrating mushroom β-glucan (Glu) from PTR and AuNRs as a novel PTT agents for cancer therapy.
In this study, AuNR-Glu with SPR peak at ~1064 nm were synthesized by a seed-mediated growth method with CTAB templates and then encapsulated with Glu into nanorod hybrids. The PTT effects of the AuNR-Glu were evaluated by in vitro and in vivo cancer cell experiments.
AuNR-Glu displayed high photothermal stability with remarkably colloidal stability in biological media and low cytotoxicity. It was effective against colon cancer cells including HT-29 and SW480 at a low dose of 20 μg/mL.The size of tumors implanted in athymic Balb/c mice were significantly reduced after an intravenous injection of AuNR-Glu (25 mg/kg body weight). No harmful side effect of AuNR-Glu was noted by histological analysis of major organs in the animals.
These results suggest that the PTR β-glucans can serve as a potential carrier of AuNRs into the colon for PTT. Such β-glucan-based encapsulation method provides new opportunities to design biocompatible functional nanomaterials for wider clinical applications.
著者Li X., Cheung P.C.K
會議名稱6th International Conference on Multifunctional, Hybrid and Nanomaterials
會議地點Stiges, Barcelona

上次更新時間 2019-26-11 於 11:57