Revealing cellular and molecular transitions in neonatal germ cell differentiation using single cell RNA sequencing
Publication in refereed journal

替代計量分析
.

其它資訊
摘要Neonatal germ cell development provides the foundation of spermatogenesis. However, a systematic understanding of this process is still limited. To resolve cellular and molecular heterogeneity in this process, we profiled single cell transcriptomes of undifferentiated germ cells from neonatal mouse testes and employed unbiased clustering and pseudotime ordering analysis to assign cells to distinct cell states in the developmental continuum. We defined the unique transcriptional programs underlying migratory capacity, resting cellular states and apoptosis regulation in transitional gonocytes. We also identified a subpopulation of primitive spermatogonia marked by CD87 (plasminogen activator, urokinase receptor), which exhibited a higher level of self-renewal gene expression and migration potential. We further revealed a differentiation-primed state within the undifferentiated compartment, in which elevated Oct4 expression correlates with lower expression of self-renewal pathway factors, higher Rarg expression, and enhanced retinoic acid responsiveness. Lastly, a knockdown experiment revealed the role of Oct4 in the regulation of gene expression related to the MAPK pathway and cell adhesion, which may contribute to stem cell differentiation. Our study thus provides novel insights into cellular and molecular regulation during early germ cell development.
出版社接受日期17.02.2019
著者LIAO JY, NG SH, LUK AC, SUEN HC, QIAN Y, LEE AWT, TU JJ, FUNG JCL, TANG NLS, FENG B, CHAN WY, FOUCHET P, HOBBS RM, LEE TL
期刊名稱Development
出版年份2019
月份3
卷號146
期次6
出版社Company of Biologists: Development
文章號碼dev174953
國際標準期刊號0950-1991
語言英式英語
關鍵詞Single cell, Transcriptomes, Spermatogonia, Gonocytes, Differentiation
Web of Science 學科類別Developmental Biology;Developmental Biology

上次更新時間 2020-02-08 於 03:08