Protection of tissue physicochemical properties using polyfunctional crosslinkers
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AbstractUnderstanding complex biological systems requires the system-wide characterization of both molecular and cellular features. Existing methods for spatial mapping of biomolecules in intact tissues suffer from information loss caused by degradation and tissue damage. We report a tissue transformation strategy named stabilization under harsh conditions via intramolecular epoxide linkages to prevent degradation (SHIELD), which uses a flexible polyepoxide to form controlled intra- and intermolecular crosslink with biomolecules. SHIELD preserves protein fluorescence and antigenicity, transcripts and tissue architecture under a wide range of harsh conditions. We applied SHIELD to interrogate system-level wiring, synaptic architecture, and molecular features of virally labeled neurons and their targets in mouse at single-cell resolution. We also demonstrated rapid three-dimensional phenotyping of core needle biopsies and human brain cells. SHIELD enables rapid, multiscale, integrated molecular phenotyping of both animal and clinical tissues.
Acceptance Date26/09/2018
All Author(s) ListYoung-Gyun Park, Chang Ho Sohn, Ritchie Chen, Margaret McCue, Dae Hee Yun, Gabrielle T Drummond, Taeyun Ku, Nicholas B Evans, Hayeon Caitlyn Oak, Wendy Trieu, Heejin Choi, Xin Jin, Varoth Lilascharoen, Ji Wang, Matthias C Truttmann, Helena W Qi, Hidde L Ploegh, Todd R Golub, Shih-Chi Chen, Matthew P Frosch, Heather J Kulik, Byung Kook Lim, Kwanghun Chung
Journal nameNature Biotechnology
Year2019
Month1
Volume Number37
Issue Number1
Place of PublicationUSA
Pages73 - 83
ISSN1087-0156
LanguagesEnglish-United States
Keywordstissue, shield

Last updated on 2020-15-10 at 03:06