Metabolomics of Green-Tea Catechins on Vascular-Endothelial-Growth-Factor-Stimulated Human-Endothelial-Cell Survival
Publication in refereed journal

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其它資訊
摘要Neovascularization causes serious oculopathy related to upregulation of vascular-endothelial-growth factor (VEGF) causing new capillary growth via endothelial cells. Green-tea-extract (GTE) constituents possess antiangiogenesis properties. We used VEGF to induce human umbilical-vein endothelial cells (HUVECs) and applied GTE, epigallocatechin gallate (EGCG), and mixtures of different compositions of purified catechins (M1 and M2) to evaluate their efficacies of inhibition and their underlying mechanisms using cell-cycle analysis and untargeted metabolomics techniques. GTE, EGCG, M1, and M2 induced HUVEC apoptosis by 22.1 ± 2, 20.0 ± 0.7, 50.7 ± 8.5, and 69.8 ± 4.1%, respectively. GTE exerted a broad, balanced metabolomics spectrum, involving suppression of the biosynthesis of cellular building blocks and oxidative-phosphorylation metabolites as well as promotion of the biosynthesis of membrane lipids and growth factors. M2 mainly induced mechanisms associated with energy and biosynthesis suppression. Therefore, GTE exerted mechanisms involving both promotion and suppression activities, whereas purified catechins induced extensive apoptosis. GTE could be a more promising antineovascularization remedy for ocular treatment.
出版社接受日期08.11.2018
著者Kai On Chu, Kwok Ping Chan, Sun On Chan, Tsz Kin Ng, Vishal Jhanji, Chi Chiu Wang, Chi Pui Pang
期刊名稱Journal of Agricultural and Food Chemistry
出版年份2018
月份12
日期5
卷號66
期次48
出版社ACS Publications
出版地USA
頁次12866 - 12875
國際標準期刊號0021-8561
電子國際標準期刊號1520-5118
語言美式英語
關鍵詞apoptosis, catechins, green-tea extract, HUVEC, metabolomics, proliferation

上次更新時間 2021-21-01 於 02:04