Metabolomics of Green-Tea Catechins on Vascular-Endothelial-Growth-Factor-Stimulated Human-Endothelial-Cell Survival
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AbstractNeovascularization causes serious oculopathy related to upregulation of vascular-endothelial-growth factor (VEGF) causing new capillary growth via endothelial cells. Green-tea-extract (GTE) constituents possess antiangiogenesis properties. We used VEGF to induce human umbilical-vein endothelial cells (HUVECs) and applied GTE, epigallocatechin gallate (EGCG), and mixtures of different compositions of purified catechins (M1 and M2) to evaluate their efficacies of inhibition and their underlying mechanisms using cell-cycle analysis and untargeted metabolomics techniques. GTE, EGCG, M1, and M2 induced HUVEC apoptosis by 22.1 ± 2, 20.0 ± 0.7, 50.7 ± 8.5, and 69.8 ± 4.1%, respectively. GTE exerted a broad, balanced metabolomics spectrum, involving suppression of the biosynthesis of cellular building blocks and oxidative-phosphorylation metabolites as well as promotion of the biosynthesis of membrane lipids and growth factors. M2 mainly induced mechanisms associated with energy and biosynthesis suppression. Therefore, GTE exerted mechanisms involving both promotion and suppression activities, whereas purified catechins induced extensive apoptosis. GTE could be a more promising antineovascularization remedy for ocular treatment.
Acceptance Date08/11/2018
All Author(s) ListKai On Chu, Kwok Ping Chan, Sun On Chan, Tsz Kin Ng, Vishal Jhanji, Chi Chiu Wang, Chi Pui Pang
Journal nameJournal of Agricultural and Food Chemistry
Volume Number66
Issue Number48
PublisherACS Publications
Place of PublicationUSA
Pages12866 - 12875
LanguagesEnglish-United States
Keywordsapoptosis, catechins, green-tea extract, HUVEC, metabolomics, proliferation

Last updated on 2021-21-01 at 02:04