Effect of quercetin on human fibroblast-like synovial cells and chondrocytes from osteoarthritis patients
Refereed conference paper presented and published in conference proceedings


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AbstractIntroduction: Osteoarthritis (OA) is a whole joint disease which involves synovial inflammation, cartilage degradation and subchondral bone remodelling. It is known that pro-inflammatory mediators induce joint inflammation and cartilage degradation. Quercetin (Que) is a dietary supplement that has both anti-inflammatory and anti-oxidative properties. In this study, the effect of Que to antagonize IL-1β induced inflammation and cartilage degradation were investigated in fibroblast-like synovial (FLS) cells and chondrocytes from OA patients.

Methodology: Human chondrocytes (passage 1) and FLS (passage 4) were stimulated with IL-1β (1 ng/mL), followed by co-incubation with Que (0.1 mM) for 24 hours. Cells were also incubated in plain medium, Que (0.1 mM) and IL-1β (1 ng/mL) as controls (n=6 patients). The mRNA expression of IL-6, COX-2, iNOS, IL-10 and HAS1 were measured in FLS, while IL-1β, COX-2, iNOS, NOX4, MMP-13, ADAMTS5, and COL2A were measured in chondrocytes. GAPDH was the housekeeping gene. Kruskal-Wallis test followed by Mann–Whitney U test was used to compare differences between groups. Statistical significance was accepted at α=0.05.

Results and Analysis: Both cell types from OA knees responded to IL-1β stimulation, with significant upregulation in most genes measured. In FLS, co-incubation with Que downregulated the mRNA expression of iNOS, HAS1 and IL-6, except COX2 and IL-10. In chondrocytes, co-incubation with Que downregulated the mRNA expression of all genes measured, except COL2A.

Discussion and Conclusion: Quercetin reduced IL-1β stimulated inflammatory and degenerative response in FLS and chondrocytes from OA knee patients. Hence, dietary supplements of Que may be a safe, alternative anti-inflammatory treatment for OA of the knee.
All Author(s) ListKW Ho, SSW Mok, SC Fu, KM Chan, J Malda, PSH Yung
Name of ConferenceThe 38th Annual Congress of the Hong Kong Orthopaedic Association
Start Date of Conference03/11/2018
End Date of Conference04/11/2018
Place of ConferenceRoom S221-S230, Hong Kong Convention and Exhibition Centre, Wan Chai, Hong Kong
Country/Region of ConferenceHong Kong
Year2018
LanguagesEnglish-United Kingdom

Last updated on 2019-12-02 at 09:29