Role of transient receptor potential vanilloid 1 channels in the regulation of calcium transients and action potentials of embryonic stem cell-derived cardiomyocytes
Refereed conference paper presented and published in conference proceedings


摘要Transient receptor potential (TRP) channels are broadly expressed in a variety of tissues and cell types and they are able to respond to a wide range of stimuli in the cellular environment. Among TRP channels, TRPV1 channel is activated by heat, pain inducers and the pungent component of hot chili peppers, capsaicin. Previous studies showed that upon stimulation, TRPV1 channel can generate intracellular Ca2+ concentration ([Ca2+]i) changes by Ca2+ entry via the plasma membrane or by Ca2+ release from intracellular organelles. As a fundamental property of cardiomyocytes (CMs), [Ca2+]i plays an important role in different cellular processes such as excitation-contraction coupling, cell proliferation and cell death. However, there is only limited knowledge on the function of TRPV1 channel in the Ca2+-handling properties and electrophysiological characteristics of CMs. In this study, we used embryonic stem cells (ESCs)-derived CMs as a model to study the role of TRPV1 channel in CMs. Our recent results revealed that TRPV1 is located on the sarcoplasmic reticulum (SR) of ESCs-derived CMs and acts as a Ca2+ release channel on the SR. In addition, activation of TRPV1 had no effect on Ca2+ sparks, which can reflect the opening of the ryanodine receptors. Functionally, TRPV1 agonist capsaicin decreased the rate and diastolic depolarization slope of action potential of ESC-derived CMs, as well as the amplitude and frequency of spontaneous Ca2+ transients of ESC-derived CMs. In the near future, to study the role of TRPV1 in ESC-derived CMs, TRPV1 will be knocked down by using adenovirus harboring shRNA against TRPV1. This study will not only give a better understanding of Ca2+ homeostasis of ESC-derived CMs, but also provide insights into the future cell replacement therapies.
著者Rui ZHAO, Zenghua QI, Xiaoqiang YAO, Suk Ying TSANG
會議名稱International Society of Stem Cell Research 2018 Annual Meeting
會議論文集題名International Society of Stem Cell Research 2018 Annual Meeting

上次更新時間 2018-12-12 於 16:32