Canonical BMP signaling determines the timing of neurogenesis and Bergmann glial development in cerebellum
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AbstractNeural stem cells (NSCs) are capable of proliferation and differentiation to generate neurons and glial cells. In mouse cerebellum, the anterior rhombic lip (ARL) and the ventricular zone (VZ) are the primary NSC pools. From embryonic day (E) 10 onwards, the VZ sequentially produces the Purkinje cells and interneurons, followed by the astrocytes. We have shown that canonical BMP signaling, which is mediated by Smad1/5/8, is activated in the NSCs at the VZ. Thus, we generated cerebellum specific Smad1/5 knockout mutants using Cre/loxP strategy to analyze the functions of canonical BMP signaling in NSC maintenance, neurogenesis and gliogenesis at cerebellar VZ. Ablation of Smad1/5 resulted in precocious induction of proneural gene expression and triggered neurogenesis prematurely. Furthermore, Smad1/5 mutants exhibited reduction in cell proliferation at the VZ and decreased production of astrocytes. Interestingly, phosphorylated Smad1/5/8 is expressed by the astrocytes within the Purkinje cell plate at late gestation. We found that the organization of Bergmann glial fibers and the monolayer arrangement of Bergmann glia somata were distorted in Smad1/5 mutants. Altogether, our findings suggest BMP/Smad signaling controls the timing of neurogenesis by regulating proneural gene expression and contributes to the development of Bergmann glia.
All Author(s) ListTC Ma, KI Vong, KM Kwan
Name of ConferenceISDN 2018: 22nd Biennial Meeting of the International Society for Developmental Neuroscience
Start Date of Conference22/05/2018
End Date of Conference25/05/2018
Place of ConferenceNara
Country/Region of ConferenceJapan
Year2018
LanguagesEnglish-United States

Last updated on 2018-25-10 at 12:22