The citrus flavonone hesperetin attenuates the nuclear translocation of aryl hydrocarbon receptor
Publication in refereed journal

香港中文大學研究人員
替代計量分析
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其它資訊
摘要The environmental polycyclic aromatic hydrocarbons (PAH) and dioxins are carcinogens and their adverse effects have been largely attributed to the activation of AhR. Hesperetin is a flavonone found abundantly in citrus fruits and has been shown to be a biologically active agent. In the present study, the effect of hesperetin on the nuclear translocation of AhR and the downstream gene expression was investigated in MCF-7 cells. Confocal microscopy indicated that 7, 12-dimethylbenz[α]anthracene (DMBA) or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) -induced nuclear translocation of AhR was deterred by hesperetin treatment. The reduced nuclear translocation could also be observed in Western analysis. Reporter-gene assay further illustrated that the induced XRE transactivation was weakened by the treatment of hesperetin. Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) assay demonstrated that the gene expressions of CYP1A1, 1A2, and 1B1 followed the same pattern of AhR translocation. These results suggested that hesperetin counteracted AhR transactivation and suppressed the downstream gene expression.
著者Yan Qin Tan, Leo Clement Chiu-Leung, Shu-mei Lin, Lai K. Leung
期刊名稱Comparative Biochemistry and Physiology - Part C: Toxicology and Pharmacology
出版年份2018
月份8
卷號210
出版社Elsevier
頁次57 - 64
國際標準期刊號1532-0456
電子國際標準期刊號1878-1659
語言英式英語

上次更新時間 2020-30-09 於 01:10