A dual genetic tracing system identifies diverse and dynamic origins of cardiac valve mesenchyme
Publication in refereed journal

香港中文大學研究人員
替代計量分析
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其它資訊
摘要In vivo genomic engineering is instrumental for studying developmental biology and regenerative medicine. Development of novel systems with more site-specific recombinases (SSRs) that complement with the commonly used Cre-loxP would be valuable for more precise lineage tracing and genome editing. Here, we introduce a new SSR system via Nigri-nox. By generating tissue-specific Nigri knock-in and its responding nox reporter mice, we show that the Nigri-nox system works efficiently in vivo by targeting specific tissues. As a new orthogonal system to Cre-loxP, Nigri-nox provides an additional control of genetic manipulation. We also demonstrate how the two orthogonal systems Nigri-nox and Cre-loxP could be used simultaneously to map the cell fate of two distinct developmental origins of cardiac valve mesenchyme in the mouse heart, providing dynamics of cellular contribution from different origins for cardiac valve mesenchyme during development. This work provides a proof-of-principle application of the Nigri-nox system for in vivo mouse genomic engineering. Coupled with other SSR systems, Nigri-nox would be valuable for more precise delineation of origins and cell fates during development, diseases and regeneration.
著者Liu K, Yu W, Tang M, Tang J, Liu X, Liu Q, Li Y, He L, Zhang L, Evans SM, Tian X, Lui KO, Zhou B
期刊名稱Development
出版年份2018
月份9
卷號145
期次18
文章號碼dev167775
國際標準期刊號0950-1991
電子國際標準期刊號1477-9129
語言英式英語
關鍵詞Genetic engineering,Site-specific recombinase (SSR),Nigri-noxFate mapping,Cardiac valve development

上次更新時間 2021-18-01 於 01:27