Functional Studies of Exocyst Positive Organelle in Plant
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AbstractExtracellular vesicles (EVs) in eukaryotic cells play important roles in mediating intercellular communication via delivering biomolecules to extracellular space. In mammalian cells, there are three kinds of EVs including 1) exosomes derived from MVB---plasma membrane (PM) fusion, 2) shedding microvesicles directly budding from the PM, and 3) apoptic bodies generated by cell death. EVs may bear proteins, RNAs and lipids. There are increasing evidences in supporting the functions of EVs in both normal physiological and pathologic processes regulation such as blood coagulation and tissue repair. In plant cells, EVs like exosomes have also been identified to play important functions in plants such as pathogen defense. Recently, we have identified a novel organelle in plant termed EXPO (exocyst positive organelle) defined by Exocyst subunit EXO70E2, under confocal microscopy, the signals of EXO70E2-XFP show puncta pattern mainly distributed along the plasma membrane which indicates the localization of EXPO organelles, EXPO may mediate an unconventional protein secretion (UPS) pathway via EXPO-PM fusion to release single membrane bound EXPO-like EVs into the apoplast, thus transporting the cytoplasmic contents across the PM. However, the function of EXPO remains elusive in plant. By using a combination of cellular, proteomic and molecular approaches, we have identified a novel EXPO potential cargo which may involve in pathogen defence pathways and giving us a hint that EXPO may involve in plant-pathogen interactions, We are now trying to conduct further test on the formation as well as cargo transportation of EXPO organelle under pathogen stress for further study on the relationships between EXPO and plant responses to pathogen attack. This research is supported by grant from the Research Grants Council of Hong Kong ( CUHK14130716, CUHK2/CRF/11G, C4011-14R, C4012-16E and AoE/M-05/12)
Acceptance Date12/11/2018
All Author(s) ListJiayang Gao, Liwen Jiang
Name of Conference2018 CSHA Meeting on “Biology of Extracellular Vesicles”
Start Date of Conference12/11/2018
End Date of Conference16/11/2018
Place of ConferenceSuzhou
Country/Region of ConferenceChina
Year2018
LanguagesEnglish-United States

Last updated on 2019-04-07 at 10:50