Analysis of Autophagic Activity Using ATG8 Lipidation Assay in Arabidopsis thaliana
Publication in refereed journal

香港中文大學研究人員
作者已離職


引用次數
替代計量分析
.

其它資訊
摘要As a fundamental metabolic pathway to degrade and recycle cellular cargos, autophagy is highly induced upon stress, starvation and senescence conditions in plants. A double-membrane structure named autophagosome will form during this process for cargo sequestration and delivery into the vacuole.
A number of regulators have been characterized in plants, including the autophagy-related (ATG) proteins and other plant-specific proteins. Among them, ATG8 will undergo a lipidation process to become a membrane-bound ATG8-phosphatidylethanolamine form and mark the growing autophagosomal membrane as well as the completed autophagosome. Therefore, ATG8 has been regarded as a marker for autophagosomes; and biochemical detection of the membrane-associated form of ATG8 is used as one of the principal methods for measurement of autophagic activity. Here, we describe an ATG8 lipidation assay for detection of the ATG8-PE form using Arabidopsis thaliana seedlings.
著者Mengqian Luo, Xiaohong Zhuang
期刊名稱Bio-protocol
出版年份2018
月份6
日期20
卷號8
期次12
出版社Bio-Protocol
國際標準期刊號2331-8325
語言美式英語
關鍵詞ATG8, ATG8-PE, Lipidation, Autophagy, Autophagosome

上次更新時間 2020-19-09 於 04:40