Investigation on the anti-metastatic activities of a triterpenoid actein in breast cancer via cell adhesion and invasion and matrix degradation
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AbstractBackground: Breast cancer is the most common type of cancer and the primary cause of cancer mortality in women. Metastasis is responsible for more than 90% breast cancer death. However, standard therapies could not diminish the ratio of metastatic breast cancer, which is always being an obstacle for clinicians. Therefore, a novel anti-metastatic drug is urgently needed. Chinese herbal medicines can be good sources of anti-metastatic agents, which may interfere the enzymatic degradation of extracellular matrix (ECM) by matrix metalloproteinases (MMPs), cancer cell motility, proliferation and adhesion to ECM. All the above play critical role in cancer invasion and metastasis. Actein, a cycloartane triterpene isolated from black cohosh and other Cimicifuga species, has been proven to inhibit the growth of breast cancer cells in vitro. However, its anti-metastatic activities in breast cancer have not been explored. In this study, we investigated the anti-metastatic effect of actein on human breast cancer cells.
Methods: Human breast cancer cells MDA-MB-231 were used in this study. Activities of MMP-2 and MMP-9 (gelatinases) of MDA-MB-231 cells were assessed by gelatin zymography. Cell motility and proliferation were detected by scratch wound healing assay and [methyl-3H]-thymidine incorporation assay, respectively. Intercellular adhesion molecule-1 (ICAM-1) expression on MDA-MB-231 cell surface, which is important for attracting immune cells, was determined by flow cytometry after stained with fluorochrome PE-conjugated monoclonal antibodies.
Results: The activities of MMP-9 of MDA-MB-231 cells were significantly suppressed by actein (20-40 μM). Besides, actein (10-40 μM) also significantly inhibited the motility and proliferation of MDA-MB-231 cells (all p<0.05). The expression of ICAM-1 on MDA-MB-231 cells increased after 48h treatment of actein.
Conclusion: In summary, our data suggest that actein could induce anti-metastatic effects on breast cancer cells via reducing cell proliferation, invasion and inhibiting matrix degradation. Further studies in breast xenograft-bearing mouse model will be used to confirm the changes of ICAM-1 expression in tumors and the anti-metastatic effect of actein.
Acceptance Date11/11/2017
All Author(s) ListXiao-Xiao WU, Grace Gar-Lee YUE, Ming-Hua QIU, Clara Bik-San LAU, Chun-Kwok WONG
Name of ConferenceHong Kong Society for Immunology 2017 Annual General Meeting and Scientific Meeting
Start Date of Conference11/11/2017
End Date of Conference11/11/2017
Place of ConferenceHong Kong
Country/Region of ConferenceHong Kong
Proceedings TitleHong Kong Immunology Forum 2017
Year2017
Month11
Day11
Pages40 - 40
LanguagesEnglish-United Kingdom

Last updated on 2018-04-12 at 17:13