Actein inhibits the invasion and migration of human breast cancer cells via cofilin pathway and suppresses metastasis in vivo
Refereed conference paper presented and published in conference proceedings


摘要Background and purpose: Breast cancer is currently the highly malignant and second most lethal cancer among women1. More than 90 % breast cancer death is due to metastasis. Anti-metastatic agents are urgently needed since standard chemotherapeutics cannot diminish the metastatic rate. Actein, a cycloartane triterpenoid isolated from black cohosh and other Cimicifuga species, has been demonstrated to be anti-angiogenic and anti-tumor2,3. The anti-metastatic activity of actein and the underlying mechanisms were evaluated in the present study.
Methods: In vivo effect of actein was examined on human breast cancer xenograft zebrafish embryos model. The in vitro effects of actein on the proliferation, cell cycle phase distribution, migration and motility were evaluated in two human breast cancer cell lines, MDA-MB-231 (estrogen receptor-negative) and MCF-7 cells (estrogen receptor-positive). Western blots and real-time PCR were employed to examine the changes of protein and mRNA expressions of relevant molecules after actein treatment.
Results: Actein (60 µM) significantly decreased the number of zebrafish embryos with migrated breast cancer cells by 74 % and reduced the number of metastatic cells in embryos. In vitro experiments showed that actein treatment (40 µM for MDA-MB-231 cells; 100 µM for MCF-7 cells) significantly decreased cell proliferation, migration and motility in MDA-MB-231 cells and MCF-7 cells, while MDA-MB-231 cells were more sensitive to actein treatment than MCF-7 cells. Furthermore, actein (40 µM) could cause G1 phase cell cycle arrest and suppress the protein expression of matrix metalloproteinases (MMPs). Actein treatment down-regulated the protein expressions of epidermal growth factor receptor (EGFR) and cofilin together with the up-regulation of related proteins and kinases in cofilin pathway.
Conclusion: Actein exhibited both in vivo and in vitro anti-metastatic activities. The underlying mechanisms of action involved the cofilin signaling pathway. Further studies in xenograft-bearing mouse model will be used to confirm the anti-metastatic effect of actein.
著者Xiao-Xiao Wu, Grace Gar-Lee Yue, Ming-Hua Qiu, Clara Bik-San Lau, Chun-Kwok Wong
會議名稱The 14th International Postgraduate Symposium on Chinese Medicine
會議地點Hong Kong
會議論文集題名The 14th International Postgraduate Symposium on Chinese Medicine
系列標題Oral presentation abstracts
頁次16 - 17

上次更新時間 2018-04-12 於 17:08