Actein inhibits the migration of human breast cancer cells via cofilin pathway and suppresses proliferation and dissemination in vivo
Refereed conference paper presented and published in conference proceedings


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摘要Background and purpose: Breast cancer is currently the highly malignant and second most lethal cancer in women. More than 90% breast cancer death is due to metastasis. Anti-metastatic agents are urgently needed since standard chemotherapeutics cannot diminish the metastatic rate. Actein, a cycloartane triterpenoid, has been demonstrated to be anti-angiogenic and anti-tumor. Its anti-migration activity and underlying mechanisms were evaluated in the present study.
Methods: In vivo effect of actein was examined on a human metastatic breast cancer cell xenograft in transparent zebrafish embryos model. Its in vitro effects on the proliferation, cell cycle phase distribution, migration and motility were evaluated using two human breast cancer cell lines, MDA-MB-231 (estrogen receptor-negative) and MCF-7 cells (estrogen receptor-positive). Western blots and real-time PCR were employed to examine the protein and mRNA expression of relevant regulatory molecules.
Results: Actein (60 µM) significantly decreased the number of zebrafish embryos with migrated cells by 74% and reduced the number of metastatic cells in embryos. In vitro experiments showed actein treatment significantly decreased cell proliferation, migration and motility. Furthermore, actein could cause G1 phase cell cycle arrest and suppress the protein expression of matrix metalloproteinases (MMPs). Actein treatment down-regulated the protein expression of epidermal growth factor receptor (EGFR) and cofilin together with the up-regulation of related proteins and kinases in cofilin pathway.
Conclusion: Actein exhibited anti-metastatic activities. The underlying mechanisms of action involved the cofilin signaling pathway. Therefore, actein could be a potential anti-metastatic natural compound for treating advanced breast cancer.
出版社接受日期15.09.2018
著者Xiao-Xiao Wu, Grace Gar-Lee Yue, Ming-Hua Qiu, Clara Bik-San Lau, Chun-Kwok Wong
會議名稱Hong Kong Immunology Forum 2018
會議開始日15.09.2018
會議完結日15.09.2018
會議地點Hong Kong
會議國家/地區香港
會議論文集題名Hong Kong Society for Immunology 2018 Annual General Meeting and Scientific Meeting
系列標題Poster Presentation
出版年份2018
月份9
日期15
頁次26 - 26
語言英式英語

上次更新時間 2018-05-12 於 09:22