Actein inhibits the migration of human breast cancer cells via cofilin pathway and suppresses proliferation and dissemination in vivo
Refereed conference paper presented and published in conference proceedings


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AbstractBackground and purpose: Breast cancer is currently the highly malignant and second most lethal cancer in women. More than 90% breast cancer death is due to metastasis. Anti-metastatic agents are urgently needed since standard chemotherapeutics cannot diminish the metastatic rate. Actein, a cycloartane triterpenoid, has been demonstrated to be anti-angiogenic and anti-tumor. Its anti-migration activity and underlying mechanisms were evaluated in the present study.
Methods: In vivo effect of actein was examined on a human metastatic breast cancer cell xenograft in transparent zebrafish embryos model. Its in vitro effects on the proliferation, cell cycle phase distribution, migration and motility were evaluated using two human breast cancer cell lines, MDA-MB-231 (estrogen receptor-negative) and MCF-7 cells (estrogen receptor-positive). Western blots and real-time PCR were employed to examine the protein and mRNA expression of relevant regulatory molecules.
Results: Actein (60 µM) significantly decreased the number of zebrafish embryos with migrated cells by 74% and reduced the number of metastatic cells in embryos. In vitro experiments showed actein treatment significantly decreased cell proliferation, migration and motility. Furthermore, actein could cause G1 phase cell cycle arrest and suppress the protein expression of matrix metalloproteinases (MMPs). Actein treatment down-regulated the protein expression of epidermal growth factor receptor (EGFR) and cofilin together with the up-regulation of related proteins and kinases in cofilin pathway.
Conclusion: Actein exhibited anti-metastatic activities. The underlying mechanisms of action involved the cofilin signaling pathway. Therefore, actein could be a potential anti-metastatic natural compound for treating advanced breast cancer.
Acceptance Date15/09/2018
All Author(s) ListXiao-Xiao Wu, Grace Gar-Lee Yue, Ming-Hua Qiu, Clara Bik-San Lau, Chun-Kwok Wong
Name of ConferenceHong Kong Immunology Forum 2018
Start Date of Conference15/09/2018
End Date of Conference15/09/2018
Place of ConferenceHong Kong
Country/Region of ConferenceHong Kong
Proceedings TitleHong Kong Society for Immunology 2018 Annual General Meeting and Scientific Meeting
Series TitlePoster Presentation
Year2018
Month9
Day15
Pages26 - 26
LanguagesEnglish-United Kingdom

Last updated on 2018-05-12 at 09:22