Hydrogen peroxide induced tendinopathic changes in a rat model of patellar tendon injury
Publication in refereed journal


摘要Tendinopathy includes cases with chronic tendon pain and spontaneous tendon ruptures, which is putatively resulted from failed tendon healing. Overuse is a major risk factor of tendinopathy, which can impose mechanical and oxidative stress to tendons. Previous studies investigated the influences of mechanical stress, but the direct impact of oxidative stress on tendon healing remains unclear. We hypothesized that imposed oxidative stress can impair tendon healing and lead to tendinopathic changes. Thirty-nine rats were operated for patellar tendon window injury. From weeks 3-5 post-operation, the rats received three weekly subcutaneous injections of saline, 50 or 500 mu M H2O2 (n = 13) over patellar tendon. Gait analysis for pain assessment and 3D ultrasound imaging for detection of tendinopathic changes were performed at pre-injury and 6-week post-operation. At week 6, knee specimens were harvested for histology or tensile mechanical test. Elastic modulus of the healing patellar tendons was significantly lower in 50 mu M but not 500 mu M H2O2 group, while ultimate mechanical stress was not significantly different across groups. Similarly, only the 50 mu M H2O2 group exhibited pain-associated gait asymmetry. Significant tendon swelling with increased tendon volume was observed in the 50 mu M H2O2 group. There were hypoechogenic changes in the tendon wound, but there was no significant difference in percentage vascularity. H2O2 impaired tendon healing and elicited tendinopathic changes, with respect to pain and structural abnormalities. Oxidative stress plays a role in the failed tendon healing of tendinopathies, and H2O2-induced failed tendon healing may serve as a good animal model to study tendinopathy.
著者Fu Sai Chuen, Yeung Man Yi, Rolf Christer, Yung Shu Hang Patrick, Chan Kai Ming, Hung Leung Kim
期刊名稱Journal of Orthopaedic Research
頁次3268 - 3274

上次更新時間 2021-14-10 於 23:46