Circulating mir-99b-5p is a marker of inflammation and structural damage on MRI in early rheumatoid arthritis
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AbstractBackground: Expression of several miRNAs occurs in the plasma and synovial fluid of patients with established rheumatoid arthritis (RA). We found that microRNA-143–3 p (miR-143–3 p), miR-145–5 p, and miR-99b-5p expression was associated with greater erosion volume in early RA (ERA)EULAR 2018-abstract no 1980). Whether these miRNAs are associated with bone erosion and joint inflammation on magnetic resonance imaging (MRI) is unknown.

Objectives: To determine whether plasma cell-free circulating miRNAs are associated with (a) bone erosion and (b) inflammation severity on MRI in patients with ERA.

Methods: 66 ERA patients were recruited at presentation for this cross-sectional study. 60 of these 66 patients (90.9%) were treatment naïve. MRI of the most severely affected wrist was performed in all patients. The degree of bone damage (i.e. erosions), bone inflammation (osteitis) and soft tissue inflammation (synovitis/tenosynovitis) was scored on MRI (a) semi-quantitatively using the Rheumatoid Arthritis MRI score (RAMRIS) for scoring the severity of erosions, bone marrow oedema, synovitis and tenosynovitis; and (b) quantitatively by measuring synovial and tenosynovial volume (mm3). The three most dysregulated miRNAs (miR-143–3 p, miR-145–5 p, and miR-99b-5p) identified in our previous ERA study were validated by TaqMan® qRT-PCR in all patients.

Results: Expression of miR-99b-5p was higher in ERA patients with erosions (1.28±0.61) on MRI than those without erosions (0.23±0.43, p<0.05). Subdivided according to mean RAMRIS synovitis score (5.69), miR-99b-5p expression was higher in ERA patients with relatively more synovitis (0.75±1.24) on MRI than those with relatively less synovitis (0.34±1.14, p<0.05). Bone marrow oedema or tenosynovitis on MRI were not found to be associated with specific miRNA expression. Expression of miR-99b-5p did, however, correlate with synovial (r=0.443, p=0.018) and tenosynovial volume (r=0.423, p=0.025) on MRI. Linear regression analysis revealed miR-99b-5p expression to be independently associated with both increased synovial volume (B=15.65, 95% CI 3.42~27.89, p=0.014) and increased tenosynovial volume (B=7.39, 95% CI 0.41~14.38, p=0.039) (Table).
All Author(s) ListJ. Yue, F. Xiao, J. Xu, P.C. Wong, E.K. Li, L.P. Tam, M. Li, L. Qin, J.F. Griffith, L.-S. Tam
Name of ConferenceAnnual European Congress of Rheumatology
Start Date of Conference13/06/2018
End Date of Conference16/06/2018
Place of ConferenceAmsterdam
Country/Region of ConferenceNetherlands
Proceedings TitleAnnals of the Rheumatic Diseases - Annual European Congress of Rheumatology
Year2018
Month6
Volume Number77
Issue NumberSupplement 2
PublisherBMJ
LanguagesEnglish-United Kingdom

Last updated on 2021-21-09 at 00:36