Capturing the interactome of newly transcribed RNA
Publication in refereed journal

替代計量分析
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其它資訊
摘要We combine the labeling of newly transcribed RNAs with 5-ethynyluridine with the characterization of bound proteins. This approach, named capture of the newly transcribed RNA interactome using click chemistry (RICK), systematically captures proteins bound to a wide range of RNAs, including nascent RNAs and traditionally neglected nonpolyadenylated RNAs. RICK has identified mitotic regulators amongst other novel RNA-binding proteins with preferential affinity for nonpolyadenylated RNAs, revealed a link between metabolic enzymes/factors and nascent RNAs, and expanded the known RNA-bound proteome of mouse embryonic stem cells. RIC K will facilitate an in-depth interrogation of the total RNA-bound proteome in different cells and systems.
著者Bao X, Guo X, Yin M, Tariq M, Lai Y, Kanwal S, Zhou J, Li N, Lv Y, Pulido-Quetglas C, Wang X, Ji L, Khan MJ, Zhu X, Luo Z, Shao C, Lim DH, Liu X, Li N, Wang W, He M, Liu YL, Ward C, Wang T, Zhang G, Wang D, Yang J, Chen Y, Zhang C, Jauch R, Yang YG, Wang Y, Qin B, Anko ML, Hutchins AP, Sun H, Wang H, Fu XD, Zhang B, Esteban MA
期刊名稱Nature Methods
出版年份2018
月份3
卷號15
期次3
出版社Nature Publishing Group
頁次213 - 220
國際標準期刊號1548-7091
電子國際標準期刊號1548-7105
語言英式英語

上次更新時間 2021-20-02 於 01:21