Methods to Analyze the Role of Progranulin (PGRN/GEP) on Cancer Stem Cell Features
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AbstractEmerging evidence suggests that tumors are hierarchically organized with a distinct subpopulation called cancer stem cells (CSCs) lying at the apex of the hierarchy. These cells are not only responsible for tumor initiation and progression but also endowed with stem cell properties, including self-renewal, chemoresistance, and tumor initiation. Although existing therapies can initially eliminate the bulk population of tumor, the stem cell properties of CSCs enable them to survive and repopulate the tumor, resulting in disease relapse. Recently, our group has shown that progranulin (PGRN/GEP) defined a hepatic cancer stem cell subpopulation in hepatocellular carcinoma. This subpopulation demonstrated enhanced ability for colony and spheroid formation, chemoresistance, and tumor initiation. In this chapter, we describe the methods used to isolate the progranulin+ subpopulation and analyze their CSC properties.
All Author(s) ListCheung P.F.Y., Cheung S.T.
All Editor(s) ListBateman A, Bennett HPJ, Cheung ST
Book titleProgranulin: Methods and Protocols
Series TitleMethods in Molecular Biology
Volume Number1806
PublisherHumana Press, New York, NY
Pages145 - 153
LanguagesEnglish-United States
KeywordsCancer stem cell, Tumor-initiating cell, Chemoresistance, Granulin-epithelin precursor, Self-renewal

Last updated on 2020-19-10 at 03:39