A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro
Publication in refereed journal

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其它資訊
摘要Natural killer (NK) cells belong to the innate immune system and are a first-line anti-cancer immune defense; however, they are suppressed in the tumor microenvironment and the underlying mechanism is still largely unknown. The lack of a consistent and reliable source of NK cells limits the research progress of NK cell immunity. Here, we report an in vitro system that can provide high quality and quantity of bone marrow-derived murine NK cells under a feeder-free condition. More importantly, we also demonstrate that siRNA-mediated gene silencing successfully inhibits the E4bp4-dependent NK cell maturation by using this system. Thus, this novel in vitro NK cell differentiating system is a biomaterial solution for immunity research.
著者Patrick Ming-Kuen Tang, Philip Chiu-Tsun Tang, Jeff Yat-Fai Chung, Jessica Shuk Chun Hung, Qing-Ming Wang, Guang-Yu Lian, Jingyi Sheng, Xiao-Ru Huang, Ka-Fai To, Hui-Yao Lan
期刊名稱Journal of Visualized Experiments
出版年份2018
月份1
期次131
文章號碼e56785
國際標準期刊號1940-087X
語言英式英語

上次更新時間 2020-14-08 於 02:17