A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro
Publication in refereed journal


摘要Natural killer (NK) cells belong to the innate immune system and are a first-line anti-cancer immune defense; however, they are suppressed in the tumor microenvironment and the underlying mechanism is still largely unknown. The lack of a consistent and reliable source of NK cells limits the research progress of NK cell immunity. Here, we report an in vitro system that can provide high quality and quantity of bone marrow-derived murine NK cells under a feeder-free condition. More importantly, we also demonstrate that siRNA-mediated gene silencing successfully inhibits the E4bp4-dependent NK cell maturation by using this system. Thus, this novel in vitro NK cell differentiating system is a biomaterial solution for immunity research.
著者Patrick Ming-Kuen Tang, Philip Chiu-Tsun Tang, Jeff Yat-Fai Chung, Jessica Shuk Chun Hung, Qing-Ming Wang, Guang-Yu Lian, Jingyi Sheng, Xiao-Ru Huang, Ka-Fai To, Hui-Yao Lan
期刊名稱Journal of Visualized Experiments

上次更新時間 2021-16-09 於 01:16