A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro
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AbstractNatural killer (NK) cells belong to the innate immune system and are a first-line anti-cancer immune defense; however, they are suppressed in the tumor microenvironment and the underlying mechanism is still largely unknown. The lack of a consistent and reliable source of NK cells limits the research progress of NK cell immunity. Here, we report an in vitro system that can provide high quality and quantity of bone marrow-derived murine NK cells under a feeder-free condition. More importantly, we also demonstrate that siRNA-mediated gene silencing successfully inhibits the E4bp4-dependent NK cell maturation by using this system. Thus, this novel in vitro NK cell differentiating system is a biomaterial solution for immunity research.
All Author(s) ListPatrick Ming-Kuen Tang, Philip Chiu-Tsun Tang, Jeff Yat-Fai Chung, Jessica Shuk Chun Hung, Qing-Ming Wang, Guang-Yu Lian, Jingyi Sheng, Xiao-Ru Huang, Ka-Fai To, Hui-Yao Lan
Journal nameJournal of Visualized Experiments
Year2018
Month1
Issue Number131
Article numbere56785
ISSN1940-087X
LanguagesEnglish-United Kingdom

Last updated on 2021-14-05 at 23:53