Differences in inflammatory gene expression and asthma risk in urban and rural children
Invited conference paper presented and published in conference proceedings


摘要Background: Children who grow up in rural areas are much less likely than urban children to develop allergic diseases such as asthma, but underlying immunological mechanisms are inconclusive.
Objective: To characterize the innate immune profiles of children living in rural China and to determine the possible mechanisms of the protection against asthma.
Methods: In our initial screening of 17,587 preschool children aged 5-8 years from Hong Kong (urban, n=3435) and County of Conghua (rural southern China, n=14,152), asthma and allergies were 2-3 times more prevalent in urban Hong Kong. We then proceeded to perform a case-control study recruiting asthmatic and non-asthmatic children from both Hong Kong (47 controls vs 32 cases) and Conghua (47 controls vs 19 cases). Blood samples were collected to examine mRNA expression of 8 innate immunity genes involved in inflammatory signaling pathways using quantitative real-time PCR. Peripheral blood mononuclear cells (PBMC) were assessed before and after lipopolysaccharide (LPS) stimulation.
Results: At baseline, all inflammatory genes we measured were expressed at significantly higher transcription levels in Hong Kong controls as compared with Conghua counterparts, such as A20 (differences between means: -1.04, 95%CI: -1.39 to -0.69, p<0.0001, ΔCt), MALT1 (-1.62, -2.14 to -1.09, p<0.0001, ΔCt) and TRAF6 (-1.28, -2.16 to -0.41, p<0.001, ΔCt). Upon LPS stimulation in vitro, both urban and rural children were able to upregulate the gene expression, but the upregulation were substantially stronger in children from the urban area, especially for TRAF6 (p<0.05, fold-change). However, there were no dramatic differences between Conghua and Hong Kong cases either with or without stimulation.
Conclusions: Living in rural environment will lead to downregulation of inflammatory gene expression, which may be an important mechanism explaining the rural protection against asthma and allergies.
著者YH Xing, CK Wong, SM Tsang, J Li, GWK Wong
會議名稱17th International Congress on Pediatric Pulmonology
會議論文集題名Pediatric Pulmonology
期次Suppl 1
頁次S88 - S89

上次更新時間 2018-30-10 於 12:11