Alternative splicing events of P53 caused by Somatic mutation in cancers
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摘要Tumor suppressor gene TP53 encodes an important protein named tumor protein p53 which play an important role in preventing the formation of tumors. Multiple protein isoforms of TP53 are abnormally expressed in cancers due to alternative splicing events. However the mechanisms of the alternative splicing is still unclear
Here we analyzed the transcriptome sequencing data of multiple cancers downloaded from TCGA database. We found that one of the TP53 isoforms which encodes an incomplete p53 protein are highly expressed in Lung, breast and prostate cancer samples. All of those samples have somatic mutations at the splicing acceptor sites in intron4. Most samples with high expression level of this isoform tends to have a higher allele frequency of this somatic  mutation. In addition, most samples with significant allele frequency imbalance show loss-of-heterozygosity and have a lower expression level of the p53 protein. However, we also found one lung cancer sample has a high allele frequency but  a high expression level of p53 protein. This sample may have other mechanisms to maintain the expression of p53
Our study suggested that integration of transcriptome sequencing data and whole genome sequencing data will provide more essential information for understanding the mechanism of Alternative splicing.
著者Dan Huang, Fu Yan Hu, Nelson Tang

上次更新時間 2018-04-07 於 12:01