Sophora flavescens protects against mycobacterial Trehalose Dimycolate-induced lung granulomas by inhibiting inflammation and infiltration of macrophages
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AbstractIntroduction: The immune system responds to Mycobacterium tuberculosis (MTB) infection by forming granulomas to quarantine the bacteria from spreading. Granulomas mediated inflammation is the major cause of lung destruction and disease transmission. Sophora flavescens (SF), an herbal medicine in China, has been demonstrated to exhibit bactericidal activities against MTB. However, its immune modulatory activities on MTB-mediated granulomatous inflammation have not been reported.
Methods: The in vitro effects and underlying mechanisms of flavonoids from Sophora flavescens (FSF) on cytokine, chemokine and adhesion molecule expression of murine alveolar macrophages (MH-S) and lung epithelial cells (MLE-12) upon stimulation with trehalose dimycolate (TDM), the most abundant lipoglycan on MTB surface, were evaluated. To investigate the in vivo anti-inflammatory activities of FSF, a TDM-induced granulomas mouse model was established to mimic TB mediated lung inflammation.
Results: In the present study, we found that flavonoids from Sophora flavescens (FSF) significantly suppressed the pro-inflammatory mediators released from in vitro mouse lung alveolar macrophages (MH-S) upon stimulation by TDM. In addition, FSF reduced adhesion molecules (LFA-1) expression on MH-S cells after TDM stimulation. Furthermore, FSF treatment on TDM-activated lung epithelial (MLE-12) cells significantly downregulated macrophage chemoattractant protein (MCP-1)/CCL2 expression, which in turn reduced in vitro migration of MH-S to MLE-12 cells. In TDM-induced mouse granulomas model, orally administrated with FSF significantly suppressed lung granulomas formation and inflammation.
Conclusion: These data collectively implicated an anti-inflammatory role of FSF on MTB-caused granulomatous inflammation, thereby providing evidence of FSF as an adjunct treatment for mycobacterial infection.
All Author(s) ListDehua Liu, Ben Chung Lap Chan, Miranda Sin-Man Tsang, Jing Zhu, Delong Jiao, Chun Kwok Wong
Name of ConferenceHong Kong Society of Flow Cytometry 23rd Annual General Meeting and Scientific Meeting
Start Date of Conference03/03/2018
End Date of Conference03/03/2018
Place of ConferenceHong Kong
Country/Region of ConferenceHong Kong
LanguagesEnglish-United Kingdom

Last updated on 2018-20-06 at 12:07