Alteration of gut microbiome by Pentaherbs formula in allergic asthmatic mice model
Refereed conference paper presented and published in conference proceedings


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AbstractBackground
A predominant Th2 immune response has been regarded as the classical pathogenesis of allergic asthma. Gut microbiome has recently been found to play an important immunological role in the activation of regulatory T regulatory cells (Tregs) and the deactivation of dendritic cells in asthma murine model, thereby regulating intestinal inflammation. These findings put forward that intervention to the microbes in the gastrointestinal tract could possibility prevent the development of allergic asthma. Our group recently reported that the Pentaherbs formula (PHF) consisting of five traditional Chinese herbal medicines, Lonicerae Flos, Menthae Herba, Phellodendri Cortex, Moutan Cortex and Atractylodis Rhizoma at w/w ratio of 2:1:2:2:2, exhibited potential protective effects on ovalbumin (OVA)-induced allergic asthmatic model by modulating the overall magnitude of inflammation.

Method
In this study, we sought to demonstrate that PHF modulates the gut microbiome and the percentage of CD4+CD25highFoxp3+ Tregs in spleen. Microbial DNA was extracted from the stool samples of the mice, and the hypervariable regions (V3-V4) of the 16S rRNA gene was sequenced using high-throughput Illumina HiSeq 2500. Analysis of the gut microbiome was performed at various taxonomy levels.

Results
Upon the oral administration of PHF, the operational taxonomic units (OUTs) in the gut were increased by 1.22-fold in OVA-induced allergic asthmatic mice. Changes in the gut microbiome composition were also observed at various taxonomic levels. While significantly reducing the abundance of the phylum Firmicutes; the 14-day PHF treatment significantly increases the abundance of putative asthma-protective Bacteroidetes in mice (all p<0.01). Notably, the numbers of Lachnospiraceae bacterium 3-2 and Clostridium leptum were significantly reduced (all p<0.05). On the other hand, the number of Clostridium sp. Culture-41 was remarkably increased upon a 14-day PHF treatment (p<0.01). Also, PHF significantly elevated the abundance of Rhodospirillales, an order that comprises the acetic-acid-producing Acetobacteraceae family.

Summary
These findings suggest that PHF can modulate the species composition and community structure of the gut microbiome in the OVA-induced allergic asthmatic model. This study also reveals that alteration in gut microbiome may potentially contribute to the immunomodulatory activities of PHF on allergic asthma.
All Author(s) ListTsang Miranda Sin-Man, Liu Dehua, Zhu Jing, Chan Helen Yau-Tsz, Sun Xiaoyu, Atli Karam, Shaw Pang Chui, Hon Ellis Kam-Lun, Leung Ping Chung, Wong Chun Kwok
Name of ConferenceHong Kong Society of Flow Cytometry 23 rd Annual General Meeting and Scientific Meeting
Start Date of Conference03/03/2018
End Date of Conference03/03/2018
Place of ConferenceHong Kong
Country/Region of ConferenceHong Kong
Year2018
LanguagesEnglish-United Kingdom

Last updated on 2018-20-06 at 12:02