The anti-inflammatory effects of Pentaherbs formula on ovalbumin-induced asthmatic mice model
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AbstractIntroduction: Allergic asthma is an inflammatory airway disease, affecting as many as 334 million people worldwide. Previous studies show that pentaherbs formula (PHF), which consists of five traditional Chinese herbal medicines, flos lonicerae, herba menthae, cortex phellodendri, cortex moutan and rhizoma atractylodis at w/w ratio of 2:1:2:2:2 exhibited therapeutic potential in treating oxazolone-induced atopic dermatitis-like mice model.

Objectives: As allergic asthma and atopic dermatitis are immunologically related to each other, this study aims at investigating the potential anti-inflammatory and immunomodulatory activities of PHF in asthmatic mice model.

Methods: Acute asthmatic mouse models were established by sensitizing the BALB/c mice with an immunogen 20 µg ovalbumin (OVA) - Al(OH)3 by i.p. administration on day 1 and day 8; followed by challenging the mice with the same immunogen on day 21, 22 and 23. PHF water extract (920 mg/kg/day) was administered to the mice by intragastrical delivery from day 1 (i.e. 14-day treatment) or from day 16 (i.e. 8-day treatment). One day after the last challenge, the airway hyper-responsiveness was measured as the enhanced pause (penh) value with Buxco Whole Body Plethysmography. Serum and bronchoalveolar larvage fluid (BALF) were collected for the determination of total IgE and OVA-specific IgE, and inflammatory cytokines and chemokine by ELISA and Multiplex assay, respectively.

Results: Upon 6.25 mg/mL and 12.5 mg/mL methacholine challenge, the penh value of the asthmatic mice under a 8-day PHF treatment is improved. Besides, the total IgE level in the serum is significantly reduced in the 14-day treatment group (all p<0.05).

Conclusions: In this study, pentaherbs formula was found to improve the airway hyper-responsiveness; reduce total IgE, OVA-specific IgE, and Th2-related and pro-inflammatory cytokines and chemokines in serum and BALF in the PHF treatment groups, indicating the potential use of PHF as a supplement for modulating allergic diseases.
All Author(s) ListTsang MSM, Zhu J, Liu D, Shaw PC, Hon EKL, Leung PC, Wong CK
Name of ConferenceThe 13th International Postgraduate Symposium on Chinese Medicine
Start Date of Conference17/08/2017
End Date of Conference17/08/2017
Place of ConferenceHong Kong Exhibition and Convention Center
Country/Region of ConferenceHong Kong
LanguagesEnglish-United Kingdom

Last updated on 2018-20-06 at 11:30