In vivo effects of Huangbo and Sinomenine on systemic lupus erythematosus using Murphy Roths Large (MRL)/lpr mice
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AbstractSystemic lupus erythematosus (SLE) is an autoimmune disease, with inflammatory disorders in skin, joints, kidneys and central nervous system. Its etiology and pathological mechanisms are still unclear, but it is believed to be affected by genes, hormones and environmental factors. Current treatments of SLE include glucocorticoid, nonsteroidal anti-inflammatory drugs, immunosuppressant drugs and biotherapeutic treatments. Huangbo is the dried bark of Phellodendron chinense Schneid, a medicinal plant used in Traditional Chinese Medicine (TCM) to remove damness, one of the features of SLE. Sinomenine is a single compound extracted from the root of Sinomenium acutum with anti-inflammatory and analgesic effect. In in vitro study, it reduces the release of proinflammatory mediators of rheumatoid arthritis an autoimmune joint disease.
In this in vivo study, Murphy Roths Large (MRL)/lpr mice is applied for the study of potential anti- inflammatory activities of Huangbo. In this study, lupus mice were divided into different groups according to their age, in order to evaluate the effect of Sinomenine and Huangbo on different disease severities of SLE. MRL/lpr mice were treated with Huangbo water extract (0.37g/kg bodyweight) by oral gavage 5 days a week for 4 weeks with water as control. For the study of Sinomenine, MRL/lpr mice were treated with sinomenine (100mg/kg bodyweight) by intraperitoneal (IP) injection 5 days a week for 2 weeks with PBS as control. To measure the anti-inflammatory activities, urine protein concentration, urine white blood cell concentration and skin condition of MRL/lpr mice were monitored weekly. The concentrations of IgG, anti- dsDNA antibodies and inflammatory cytokines in sera were measured by ELISA and cytometric bead array (CBA) assays with flow cytometry. Spleenocytes and thymuic cells were purified for the analysis of B cells and double negative T cells using flow cytometry.
Results showed that Sinomenine reduced the serum IgG level in mild and moderate groups of MRL/lpr mice, and the reduction is statistically significant in the mild group (p<0.05). For the flow cytometry of splenocytes, sinomenine treated mice showed a trend of higher B cells and double negative T cells percentage than control in all three age groups. Further investigations are ongoing for further evaluation of the Sinomenine and Huangbo.
All Author(s) ListYau Tsz CHAN, Miranda Sin Man TSANG, Jing ZHU, Dehua LIU, Xiaoyu SUN, Ping Chung LEUNG, Lai Shan TAM, Chun Kwok WONG
Name of ConferenceHong Kong Society for Immunology - 2017 Annual Scientific Meeting
Start Date of Conference11/11/2017
End Date of Conference11/11/2017
Place of ConferenceHong Kong
Country/Region of ConferenceHong Kong
LanguagesEnglish-United Kingdom

Last updated on 2018-20-06 at 11:49