The ethanol extract of Sophora flavescens protects against trehalose dimycolate induced lung granulomas by inhibiting inflammation and infiltration of macrophages
Refereed conference paper presented and published in conference proceedings


摘要The immune system responds to Mycobacterium tuberculosis (MTB) infection by forming granulomas to quarantine the bacteria from spreading. Granulomas mediated inflammation is a cause of lung destruction and disease transmission. Sophora flavescens (SF), an herbal medicine in China, has been demonstrated to exhibit bactericidal activities against MTB. However, its immune modulatory activities on MTB-mediated granulomatous inflammation have not been reported. In the present study, we found that flavonoids from Sophora flavescens (FSF) significantly suppressed the pro-inflammatory mediators release from mouse lung alveolar macrophages (MH-S) upon stimulation by trehalose dimycolate (TDM), the most abundant lipoglycan on MTB surface. In addition, FSF reduced adhesion molecules (LFA-1) expression on MH-S cells after TDM stimulation. Furthermore, FSF treatment on TDM-activated lung epithelial (MLE-12) cells significantly downregulated macrophage chemoattractant protein (MCP-1) expression, which in turn reduced in vitro migration of MH-S to MLE-12 cells. In TDM-induced mouse granulomas model, orally administrated with FSF significantly suppressed lung granulomas formation and inflammation. These data collectively implicating an anti-inflammatory role of FSF on MTB-caused granulomatous inflammation, thereby providing evidence of FSF as an adjunct treatment for mycobacterial infection.
著者Dehua LIU, Ben Chung Lap CHAN, Miranda Sin-Man TSANG, Jing ZHU, Ping Chung LEUNG, Chun Kwok WONG
會議名稱Hong Kong Society for Immunology - 2017 Annual Scientific Meeting
會議地點Hong Kong

上次更新時間 2018-20-06 於 11:53