Activated Osteocyte Dendrite Proliferation In Enhanced Osteoporotic Metaphyseal Fracture Healing In Response To Low-Magnitude High-Frequency Vibration
Refereed conference paper presented and published in conference proceedings


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AbstractIntroduction: Osteoporotic fracture has a high prevalence among the elderly with impaired healing properties and were mostly concentrated in metaphyseal region. Osteocytes are responsible for mechano-sensing and initiate the repair mechanism during the healing cascade. Low-magnitude high-frequency vibration (LMHFV) is a non-invasive biophysical intervention reported to promote osteoporotic fracture healing.
Objective: We hypothesized that mechanical loading altered the Osteocyte Lacuno-canalicular Network (LCN) in the ovariectomized metaphyseal fracture model.
Methods: For osteoporosis development, 72 bilateral ovariectomies were performed. A metaphyseal fracture was created and fixed. Rats were randomized to either LMHFV or control groups. Weekly radiographs were taken at week 1, 2, 6 post-fracture and MicroCT was used to evaluate the change of bone properties at fracture sites. Undecalcified histology was used to examine the LCN system both qualitatively and quantitatively by Scanning Electron and Confocal-Imaris (*p<0.05).
Results: Serial radiographies and micro CT showed an improvement of healing after the treatment of LMHFV. Morphological assessments of osteocytes revealed that the LCN exhibited a more vibrant morphology after vibration with a 11% increase in dendritic branch points, 26% increase in dendritic length at week 2 (p=0.02) and an 50% increase in length at week 6.
Conclusion: Our findings revealed that vibration promoted the rate of healing in the osteoporotic metaphyseal model, and this is the first report to indicate that there was a better healing outcome with re-vitalized morphological changes in the osteocytes. The dramatic alterations suggested that osteocyte could respond to mechanical stimulation in the form of LMHFV and might play a role in accelerating osteoporotic fracture healing.
Acceptance Date11/04/2018
All Author(s) ListMHV CHOY, RMY WONG, MC LI, SKH CHOW, JCY CHENG, WH CHEUNG
Name of ConferenceThe 4th Asia-Pacific Bone & Mineral Research Meeting and Osteoporotic Fracture Prevention & Treatment Conference 2018
Start Date of Conference11/05/2018
End Date of Conference13/05/2018
Place of ConferenceHong Kong
Country/Region of ConferenceHong Kong
Proceedings TitleThe Proceeding of The 4th Asia-Pacific Bone & Mineral Research Meeting and Osteoporotic Fracture Prevention & Treatment Conference 2018
Year2018
Month5
Place of PublicationHong Kong
Pages37 - 37
LanguagesEnglish-United Kingdom

Last updated on 2018-12-06 at 15:29