Neobavaisoflavone from Psoralea Corylifolia L. Induces Osteogenic Phenotype in Chondrogenic Cells with Potential of Enhancing Bone Healing
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AbstractBackground: Bone formation involves the participation of chondrocytes to perform chondrogenesis and subsequent osteogenesis. Estrogen and bone morphogenetic protein 2 (BMP2) are small molecules and protein that promote chondrogenesis. Osteoblastic transformation occurs after chondrogenesis, in which osteogenic markers, such as collagen type 1 (COL1), osteocalcin (BGLAP2) and osterix (OSX) are upregulated. Psoralea corylifolia L. is a traditional Chinese medicine used to treat bone injuries. Neobavaisoflavone (NBIF) is one of the main phytochemicals in Psoralea corylifolia L. In this study, we aim to investigate the in vitro effect of NBIF on the chondro-osteogenesis.

Methods: The mouse teratocarcinoma ATDC5 cell line was used as the in vitro chondrogenic model. ATDC5 cells were differentiated using 1% ITS in DMEM/F12 medium for 21 days in 5% CO2. After that, the cells were cultured in αMEM for 7 days in 3% CO2 to undergo osteogenesis and mineralization. NBIF at concentrations of 6.25, 12.5 and 25 μM were added to αMEM, while αMEM with 1% ITS was served as the control. Alizarin red-S staining was used to investigate alkaline phosphatase activity during in vitro mineralization process. Real time PCR was used to investigate the expression of chondrogenic and osteogenic markers. One-way ANOVA post hoc Dunnett’s test was used to compare the difference in mRNA expressions between the NBIF groups and control. A p value lower than 0.05 is considered as statistically significant.

Results: Calcium deposition was enhanced in chondrogenic cells treated with concentrations 12.5 and 25 μM NBIF. Under the treatment of NBIF at 25 μM, chondrogenic markers SOX9 and collagen type X A1 (COL10A1) were significantly upregulated by 1.53 (p < 0.01) and 1.37 (p < 0.01) folds compared with control. Intriguingly, osteogenic markers BGLAP2, RUNX2, COL1A1, COL1A2 and BMP2 were also significantly upregulated by 2.07 (p < 0.05), 1.88 (p < 0.01), 1.47 (p < 0.05), 1.05 (p < 0.05) and 3.80 folds (p < 0.05).

Discussion and Conclusion: NBIF has been demonstrated to be potent in promoting transdifferentiation of chondrogenic cells into osteogenic phenotype. Facilitated chondro-osteogenesis is beneficial for bone fracture healing. Further studies are required to investigate the effect of NBIF on bone formation in vivo.
All Author(s) ListAngus Yiu-Ting CHOI, Jia Wen XIAN, Zhong Rong ZHANG, Chun Wai CHAN
Name of ConferenceThe 10th Pong Ding Yuen International Symposium on Traditional Chinese Medicine
Start Date of Conference01/12/2017
End Date of Conference03/12/2017
Place of ConferenceHong Kong
Country/Region of ConferenceHong Kong
Year2017
LanguagesEnglish-United Kingdom

Last updated on 2018-04-06 at 11:59