Oncogenicity Comparison of Human Papillomavirus Type 52 E6 Prototype and Variants
Refereed conference paper presented and published in conference proceedings


摘要Cervical cancer is the fourth most common cancer affecting women worldwide, almost all cases of which are caused by human papillomavirus (HPV) infection, with HPV-16 and HPV-18 accounting for nearly 70% of cases. Different HPV types may have different global distribution patterns. The oncogenicity of HPV is attributed chiefly to the activities of the E6 and E7 oncoproteins. In this study, we focus on HPV-52 due to its unexpectedly high prevalence among Eastern Asian populations. From our previous studies, it was revealed that different HPV-52 variants might display different oncogenicities. Now that the oncogenic roles of HPV-52 E6 and E7 have not been well-studied, we carried out research to compare the differences of oncogenicity between HPV-52 E6 prototype and its variants through molecular and phenotypic approaches. We studied the capabilities of HPV-52 E6 variants to form complexes with p53 and E6AP, together with their abilities to degrade tumour suppressors, including p53, and PSD95/Dlg/ZO-1(PDZ) proteins such as Dlg and MAGI. From our preliminary results, HPV-52 E6 prototype and variants can degrade p53, Dlg and MAGI, with no significant differences being observed. Intriguingly, it was observed that HPV-52 E6 can degrade p53 better than HPV-16 E6 in H1299 cells. For p53 and E6AP binding, HPV-52 E6 variants can bind to both proteins as efficiently as HPV-52 E6 prototype and HPV-16 E6. However, it remains unclear whether HPV-52 E6 variants may have differences in exerting oncogenicities through other molecular pathways, and their transforming abilities remain to be further studied. Our findings shall provide a clearer image of oncogenicity of HPV-52 E6 variants, which is important for advanced HPV vaccine designs and clinical management, especially for the Eastern Asian populations.
著者Lai LTO, Boon SS, Law PTY, Thomas M, Banks L, Chan PKS
會議名稱INFECTION 2017
會議地點Hong Kong
會議論文集題名INFECTION 2017

上次更新時間 2018-17-05 於 16:04