Comparing Oncogenicity of Human Papillomavirus Type 52 E6 Prototype and Variants
Refereed conference paper presented and published in conference proceedings


摘要Human Papillomavirus(HPV) infection has been proven to attribute to virtually all cases of cervical cancer, which is the fourth most common cancer affecting women worldwide, with HPV-16 and -18 responsible for approximately 70% cases. HPV’s oncogenicity is ascribed mainly by E6 and E7 oncoproteins. Different global distribution patterns may be observed for various HPV types. In this study, we focus on HPV-52 due to its unexpectedly high prevalence among Eastern Asian population. Our previous studies revealed that different HPV-52 variants might display dissimilar oncogenicities, and the oncogenic roles of HPV-52 E6 and E7 have not been well-studied. Therefore, we carried out investigations comparing the oncogenicity of HPV-52 E6 prototype with its variants through molecular and phenotypic approaches. We studied HPV-52 E6 variants’ abilities in degrading p53, and Psd95/Dlg/ZO-1(PDZ) proteins like hDlg and MAGI, and their complex-forming capabilities with p53 and E6AP. From preliminary results, HPV-52 E6 prototype and variants can degrade p53, hDlg and MAGI, with no significant difference being observed. Intriguingly, we observed that HPV-52 E6 can degrade p53 better than -16 E6 in H1299 cells. HPV-52 E6 variants can form complexes with p53 and E6AP as efficient as HPV-52 E6 prototype and -16 E6. However, it is unclear whether HPV-52 E6 variants can exert their oncogenicities through other molecular pathways, and their transforming abilities remain to be further investigated. Our finding shall provide a clearer image of HPV-52 E6 variants’ oncogenicities, which is important for advanced HPV vaccine designs and clinical management, especially for the Eastern Asian population.
著者Lai LTO, Boon SS, Law PTY, Thomas M, Banks L, Chan PKS
會議名稱DNATV DNA Tumour Virus 2017
會議論文集題名DNATV Tumour Virus 2017 Birmingham,UK
頁次144 - 144
關鍵詞Human Papillomavirus (HPV), Oncogenicity, E6 oncoproteins

上次更新時間 2018-17-05 於 15:40